Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
Author:   Bradley J. Till ,  Joanna Jankowicz-Cieslak ,  Owen A. Huynh ,  Mayada M. Beshir
Publisher:   Springer International Publishing AG
Edition:   Softcover reprint of the original 1st ed. 2015
ISBN:  

9783319386676


Pages:   38
Publication Date:   17 October 2016
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
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Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols


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Author:   Bradley J. Till ,  Joanna Jankowicz-Cieslak ,  Owen A. Huynh ,  Mayada M. Beshir
Publisher:   Springer International Publishing AG
Imprint:   Springer International Publishing AG
Edition:   Softcover reprint of the original 1st ed. 2015
Dimensions:   Width: 15.50cm , Height: 0.30cm , Length: 23.50cm
Weight:   0.097kg
ISBN:  

9783319386676


ISBN 10:   3319386670
Pages:   38
Publication Date:   17 October 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

​Introduction.- Health and Safety Considerations.- Sample Collection and Storage.- Low-Cost DNA Extraction.- PCR Amplification for Low-Cost Mutation Discovery.- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples.- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants.- Example Data.- Conclusions. 

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