Overview

This volume assembles a broad spectrum of methods used in long non-coding RNAs (lncRNA) research, ranging from computational annotation of lncRNA genes to molecular and cellular analyses of the function of individual lncRNA. Long Non-Coding RNAs: Methods and Protocols also discusses methods used to study circular RNAs and RNA splicing, as well as influential findings on lncRNA in human diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Long Non-Coding RNAs: Methods and Protocols is a must-have for molecular biologists, cell and developmental biologists, specialists who conduct disease-oriented research, and bioinformatics experts who seek a better understanding on lncRNA expression and function by computational analysis of the massive sequencing data that are rapidly accumulating in recent years.

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9781493933761

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Author Information

Lin Zhang, MD Center for Research on Reproduction and Women's Health University of Pennsylvania School of Medicine BRB II/III, 421 Curie Blvd. Rm 1324-1326 and 1343 (Lab), Rm 1209 (Office) Philadelphia, PA 19104 Yi Feng Department of Cancer Biology University of Pennsylvania School of Medicine Rm 625 BRB II/III, 421 Curie Blvd. Philadelphia, PA 19104

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