Overview

This updated book explores a wide repertoire of tools and approaches that have been created, modified, and applied to the study of L. monocytogenes, forming the basis of our understanding of the bacterium today. Many of these key experimental techniques are gathered together herein. The volume presents aspects such as clinical disease and host-pathogen interactions, as well as the study of biofilms which present a significant challenge for control of the organism in the food processing environment. The topics covered in this edition also include sampling in order to isolate Listeria, methods for their identification and characterization, methods for gene manipulation, and methods for control of the organism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshootingand avoiding known pitfalls.  Authoritative and up-to-date, Listeria monocytogenes: Methods and Protocols, Second Edition aims to contribute toward the harmonization of methods used to study this important bacterium, and to be of particular interest to Listeria research both in relation to food association and control as well as clinical microbiology.

Full Product Details

Author:   Edward M. Fox ,  Hélène Bierne ,  Beatrix Stessl
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2021
Volume:   2220
Weight:   0.714kg
ISBN:  

9781071609811

ISBN 10:   1071609815
Pages:   260
Publication Date:   26 September 2020
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Detection, Quantification, and Confirmation   1. Traditional Methods of Analysis for Listeria monocytogenes             Rui Magalhães, Cristina Mena, Vânia Ferreira, Gonçalo Almeida, Joana Silva, and Paula Teixeira   2. MALDI-ToF MS: A Rapid Methodology for Identifying and Subtyping Listeria monocytogenes             Snehal R. Jadhav, Rohan M. Shah, and Enzo A. Palombo   3. Sample Preparation for qPCR Detection of Listeria from Food             Patrick Mester, Anna Kristina Witte, and Peter Rossmanith   4. qPCR Validation on the Basis of the Listeria monocytogenes prfA Assay             Anna Kristina Witte, Patrick Mester, and Peter Rossmanith   Part II: Subtyping Approaches   5. Serotype Assignment by Sero-Agglutination, ELISA, and PCR             Lisa Gorski   6. Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes             Karen Hunt and Kieran Jordan   7. Multi-Locus Sequence Typing (MLST) and Whole Genome Sequencing (WGS) of Listeria monocytogenes and Listeria innocua             Beatrix Stessl, Martin Wagner, and Werner Ruppitsch   Part III: Genotypic and Phenotypic Characterization   8. High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray             Laura Luque-Sastre, Kieran Jordan, Séamus Fanning, and Edward M. Fox   9. High-Throughput Screening of Biofilm Formation of Listeria monocytogenes on Stainless Steel Coupons Using a 96-Well Plate Format             Jessica A. Gray, P. Scott Chandry, John P. Bowman, and Edward M. Fox   10. Confocal Laser Microscopy Analysis of Listeria monocytogenes Biofilms and Spatially Organized Communities             Maud Darsonval, Marina Grégoire, Julien Deschamps, and Romain Briandet   11. Extraction and Preparation of Listeria monocytogenes Subproteomes for Mass Spectrometry Analysis             Tiago Santos and Michel Hébraud   Part IV: Strain Manipulation   12. Extraction and Analysis of Plasmid DNA from Listeria monocytogenes             Aidan Casey and Olivia McAuliffe   13. Generation of Nonpolar Deletion Mutants in Listeria monocytogenes Using the “SOEing” Method             Kathrin Rychli, Eva Harter, Caitriona M. Guinane, Karen Daly, Colin Hill, and Paul D. Cotter   14. Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes             Cameron Parsons, Reha Azizoglu, Driss Elhanafi, and Sophia Kathariou   Part V: Host-Pathogen Interactions   15. Internalization Assays for Listeria monocytogenes             Andreas Kühbacher, Pascale Cossart, and Javier Pizarro-Cerdá   16. Microscopy of Intracellular Listeria monocytogenes in Epithelial Cells             Hélène Bierne, Mounia Kortebi, and Natalie Descoeudres   Part VI: Control Methods   17. Control of Listeria monocytogenes Biofilms in a Simulated Food Processing Environment             Sofia V. Poimenidou, Stavros G. Manios, and Panagiotis N. Skandamis   18. Sampling the Food Processing Environment: Taking up the Cudgel for Preventive Quality Management in Food Processing (FP)             Martin Wagner and Beatrix Stessl   19. Isolation and Evaluation of Anti-Listeria Lactococcus lactis from Vegetal Sources             Van Thi Thuy Ho, Anran Dong, Raquel Lo, and Mark S. Turner

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