Overview

This third edition provides new and updated chapters detailing preparation of liposomes, physicochemical characterization of liposomes, lipid analysis, drug encapsulation, surface modification, stimuli response as well as cellular interaction, and biodistribution.  Also included is an updated chapter on the history and evolution of the field of liposomology. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.  Authoritative and cutting-edge, Liposomes: Methods and Protocols, Third Edition aims to serve as a reference for graduate students, post-doctoral researchers as well as established investigators utilizing lipid-based systems.

Full Product Details

Author:   Gerard G.M. D'Souza ,  Hongwei Zhang
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   3rd ed. 2023
Volume:   2622
Weight:   0.801kg
ISBN:  

9781071629536

ISBN 10:   1071629530
Pages:   305
Publication Date:   14 February 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. From olive oil emulsions to COVID-19 Vaccines - Liposomes Came First  Volkmar Weissig    2 Preparation of DRV liposomes Sophia G. Antimisiaris   3 Preparation of small unilamellar vesicles using detergent dialysis method  Qingyue Zhong and Hongwei Zhang    4 Thin-film hydration followed by extrusion method for liposome preparation  Hongwei Zhang    5 Ethanol injection method for liposome preparation  Guangsheng Du and Xun Sun   6 Preparation of giant vesicles with mixed single-tail and double-tail lipids  Lauren A Lowe and Anna Wang    7 Scalable Liposome Synthesis by High Aspect Ratio Microfluidic Flow Focusing  Jung Yeon Han, Zhu Chen, and Don L. DeVoe   8 Preparation of doxorubicin liposomes by remote loading method Jian Chen   9 Magnetic thermosensitive liposomes loaded with Doxorubicin  Mohamad Alawak, Alice Abu Dayyih, Ibrahim Awak, Bernd Gutberlet, Konrad Engelhardt, and Udo Bakowsky   10 Preparation and physical characterization of DNA binding cationic liposomes Vaibhav Saxena   11 Tunable pH sensitive lipoplexes Helene Dhotel, Michel Bessodes and Nathalie Mignet   12 Solid Lipid Nanoparticles for Drug Delivery  Wei-Chung Luo and Xiuling Lu    13 Stable Discoidal Bicelles – Formulation, Characterization and Functions   Ying Liu, Yan Xia, Armin Tahmasbi Rad, Wafa Aresh, Justin M. Fang, and Mu-Ping Nieh   14 The post-insertion method for the preparation of PEGylated liposomes Sherif E. Emam, Nehal E. Elsadek, Taro Shimizua, and Tatsuhiro Ishidaa   15 Click chemistry for liposome surface modification  Maria Vittoria Spanedda, Marcella De Giorgi, Béatrice Heurtault, Antoine Kichler, Line Bourel-Bonnet and Benoît Frisch   16 Surface Modification of Liposomes Using Folic Acid  Mengran Guo, Zhongshan He, Xi He and Xiangrong Song   17 Preparation and Characterization of Trastuzumab Fab conjugated liposomes (immunoliposomes) Yuhong Xu   18 Pyrophosphorylated cholesterol modified bone-targeting liposomes formulation procedure  Yanzhi Liu, Zhenshan Jia, Luoyang Ma, and Dong Wang   19 Method of simultaneous analysis of liposome components using HPTLC/FID Sophia Hatziantoniou and Costas Demetzos   20 HPLC-MS/MS Method for Identification and Quantification of Lipids in Liposomes  Yujie Shi and Xiaona Li   21 DPH probe method for liposome-membrane fluidity determination  Wei He   22 Imaging of Liposomes by Negative Staining Transmission Electron Microscopy and Cryogenic Transmission Electron Microscopy  Anand S. Ubhe    23 Visualization and characterization of liposomes by Atomic Force Microscopy (AFM)  Konrad Engelhardt, Eduard Preis and Udo Bakowsky   24 Determination of the sub-cellular distribution of fluorescently labeled liposomes using confocal microscopy  Melani A. Solomon    25 Liposome biodistribution via europium complexes Nathalie Mignet and Daniel Scherman   26 Quantification of a fluorescent lipid DOPE-NBD by an HPLC method in biological tissue: application to study liposome’s uptake by human placenta  Louise Fliedel, Nathalie Mignet, Thierry Fournier, Karine Andrieux, and Khair Alhareth

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