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Author:   Jose M. Ordovas
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1998 ed.
Volume:   110
Dimensions:   Width: 15.50cm , Height: 2.00cm , Length: 23.50cm
Weight:   1.360kg
ISBN:  

9780896034204

ISBN 10:   0896034208
Pages:   284
Publication Date:   25 August 1998
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Awaiting stock  
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Table of Contents

DNA- and RNA-Based Methodolog.- PCR Methodology Applied to Genetic Studies of Lipoprotein Metabolism and Atherosclerosis.- Separation of Small-Size DNA Fragments Using Agarose Gel Electrophoresis.- Quantification of mRNA by Polymerase Chain Reaction (PCR) Using an Internal Standard and a Nonradioactive Detection Method.- Generation of Transgenic Mice.- Preparation of Knockout Mice.- Protein-Based Methodology.- Fast Ultracentrifugation Methods for the Separation of Plasma Lipoproteins.- Preparative Isolation of Plasma Lipoproteins Using Fast Protein Liquid Chromatography (FPLC).- Separation of Apolipoproteins by Polyacrylamide Gel Electrophoresis.- Isolation and Purification of Serum Lipoprotein (a) Using Lectin Affinity.- Electrophoretic Separation of LDL and HDL Subclasses.- Measurement of Lipoprotein Particles.- Methodological Approaches for Assessing Lipid and Protein Oxidation and Modification in Plasma and Isolated Lipoproteins.- Separation and Quantitation of Phospholipid Classes by HPLC.- Assays of Lecithin Cholesterol Acyltransferase (LCAT).- Determination of the Mass Concentration and the Activity of the Plasma Cholesteryl Ester Transfer Protein (CETP).- In Vitro Measurement of Lipoprotein and Hepatic Lipases.- Cellular Assays for Lipoprotein Receptors.- Measurements of Proteoglycan—Lipoprotein Interaction by Gel Mobility Shift Assay.

Reviews

This text makes a good selection with admirable breadth and a high general quality of method description. -Clin Chem Lab Med The book presents the state-of-art of molecular biology and protein techniques specific for lipoprotein research...The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists... -Nahrung Each chapter contains, besides a description of the principle of the methods, highly instructive protocols and lists of the technical requirements and reagents. Notes with additional information, e.g. special experimental conditions or safety and technical advises as well as numerous references are given. The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists. -Nahrung

This text makes a good selection with admirable breadth and a high general quality of method description. - Clin Chem Lab Med The book presents the state-of-art of molecular biology and protein techniques specific for lipoprotein research...The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists... - Nahrung Each chapter contains, besides a description of the principle of the methods, highly instructive protocols and lists of the technical requirements and reagents. Notes with additional information, e.g. special experimental conditions or safety and technical advises as well as numerous references are given. The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists. - Nahrung

This text makes a good selection with admirable breadth and a high general quality of method description. -Clin Chem Lab Med The book presents the state-of-art of molecular biology and protein techniques specific for lipoprotein research...The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists... -Nahrung Each chapter contains, besides a description of the principle of the methods, highly instructive protocols and lists of the technical requirements and reagents. Notes with additional information, e.g. special experimental conditions or safety and technical advises as well as numerous references are given. The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists. -Nahrung

"""This text makes a good selection with admirable breadth and a high general quality of method description.""-Clin Chem Lab Med ""The book presents the state-of-art of molecular biology and protein techniques specific for lipoprotein research...The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists...""-Nahrung ""Each chapter contains, besides a description of the principle of the methods, highly instructive protocols and lists of the technical requirements and reagents. Notes with additional information, e.g. special experimental conditions or safety and technical advises as well as numerous references are given. The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists.""-Nahrung"

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