Overview

This volume provides the most widely used protocols and techniques employed in lipopolysaccharide( LPS) transport studies. Chapters detail identification and analysis of essential genes and crucial mutations in LPS transport-related genes, LPS profile determination, LPS chemical analysis, protein-LPS interaction, and LPS visualization. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Lipopolysaccharide Transport: Methods and Protocols aims to deliver an overview on the multidisciplinary study of LPS transport and to identify new and innovative antibacterial strategies.

Full Product Details

Author:   Paola Sperandeo
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2548
Weight:   0.772kg
ISBN:  

9781071625804

ISBN 10:   1071625802
Pages:   292
Publication Date:   25 September 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

PART I       Genetic Strategies to Study Essential LPS Biogenesis Genes 1.          Use of Mutagenesis and Functional Screens to Characterize Essential Genes Involved in Lipopolysaccharide Transport Andrew Wilson, Carlos Iniguez, and Natividad Ruiz   2.          Generation of Stable and Unmarked Conditional Mutants in Pseudomonas aeruginosa Alessandra Lo Sciuto, Maria Concetta Spinnato, Martina Pasqua, and Francesco Imperi   3.          Analysing the Function of Essential Genes by Plasmid Shuffling           Federica Anna Falchi   PART II      Functional Analysis of Proteins Involved in LPS Transport 4.          Membrane Fractionation by Isopycnic Sucrose Density Gradient Centrifugation for Qualitative Analysis of LPS in Escherichia Coli Elisabete C. Cardoso Mendes Moura, Alessandra Polissi, and Paola Sperandeo   5.          Exploring The Topology of Cytoplasmic Membrane Proteins Involved in Lipopolysaccharide Biosynthesis by in silico and Biochemical Analyses Julia Monjarás Feria and Miguel A. Valvano   6.          Use of Site‐Directed Spin Labeling EPR Spectroscopy to Study Protein–LPS Interactions Kathryn M. Schultz and Candice S. Klug   7.          A Photo-Crosslinking Approach to Monitor Assembly of an LptD Intermediate with LptE in a Living Cell Ryoji Miyazaki, Hiroyuki Mori, and Yoshinori Akiyama   8.          Mass Spectrometry Analysis of Dynamics and Interactions of the LPS translocon LptDE Francesco Fiorentino and Jani R. Bolla   9.          Affinity Purification and Co-Immunoprecipitation of Trans-envelope Protein Complexes in Gram-negative Bacteria Alessandra M. Martorana, Carlo Santambrogio, and Alessandra Polissi   10.       A Bacterial Two-Hybrid System for in vivo assays of Protein-Protein Interactions and Drug Discovery Daniel Ladant   11.       Detecting Lipoproteins Sneaking out of the Lipopolysaccharide Leaflet Naemi Csoma, Didier Colau, and Jean-François Collet   PART III     Tools for Structural Analysis of LPS and LPS Transport Proteins 12.       Dissecting Lipopolysaccharide Composition and Structure by GC-MS and MALDI Spectrometry Pilar Garcia-Vello, Immacolata Speciale, Flaviana Di Lorenzo, Antonio Molinaro, and Cristina De Castro   13.       Probing the Functional Interaction Interface of Lipopolysaccharide and Antimicrobial Peptides: A Solution-State NMR Perspective Karishma Biswas and Anirban Bhunia   14.       Cryo-EM Analysis of the Lipopolysaccharide Flippase MsbA François A. Thélot and Maofu Liao   15.       Protein Crystallization of Two Recombinant Lpt Proteins Michela Bollati and Louise J. Gourlay   PART IV    Microscopy-Based Approaches 16.       Metabolic Incorporation of Azido-Sugars into LPS to Enable Live Cell Fluorescence Imaging Inga Nilsson and David A. Six   17.       Use of Atomic Force Microscopy to Characterize LPS Perturbations           Yoojin Oh

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