Overview

This fully updated book presents an account of areas of utility, techniques, and bioinformatic advancements in the field of lipidomics. Beginning with protocols for lipid isolation and extraction, the volume continues with techniques from extractive mass spectrometry to imaging mass spectrometry methods allowing localization of lipids in tissues. These protocols have been complemented by methods addressing specific problems from membranes, fractionated subcellular compartments or organelles to whole organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Lipidomics: Methods and Protocols, Second Edition serves as an ideal guide for biochemists, molecular biologists, neuroscientists, vision research scientists, as well as all biomedical researchers with interest in disease discovery and drug development.

Full Product Details

Author:   Sanjoy K. Bhattacharya
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2023
Volume:   2625
Weight:   0.932kg
ISBN:  

9781071629659

ISBN 10:   1071629654
Pages:   374
Publication Date:   19 January 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Isolation of Mitochondrial Lipids and Mass Spectrometric Analysis             Alexa M. Jauregui, Zoé M. Cubero Cortés, Sean D. Meehan, and Sanjoy K. Bhattacharya   2. Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation             Blake R. Hopiavuori, Dustin R. Masser, Joseph L. Wilkerson, Richard S. Brush, Nawajes A. Mandal, Robert E. Anderson, and Willard M. Freeman   3. Multi-Macromolecular Extraction from Endosymbiotic Anthozoans             Anderson B. Mayfield   4. High Resolution Liquid Chromatography-Mass Spectrometry for Lipidomics             Faith Christine Harvey, Vanessa Collao, and Sanjoy K. Bhattacharya   5. Characterization of Gangliosides from Mouse Optic Nerve Samples Using Mass Spectrometry             Jennifer Arcuri and Sanjoy K. Bhattacharya   6. Protocol and Methods Applicable to Retinal Vascular Diseases             Saurabh Kumar, Manjunath B. Joshi, and Inderjeet Kaur   7. Analysis and Annotation of Phospholipids by Mass Spectrometry-Based Metabolomics             Álvaro González-Domínguez, María Santos-Martín, Ana Sayago, Alfonso María Lechuga-Sancho, Ángeles Fernández-Recamales, and Raúl González-Domínguez   8. Profiling the Mammalian Lipidome by Quantitative Shotgun Lipidomics             Mads Møller Foged, Kenji Maeda, and Mesut Bilgin   9. Lipidomics Analysis with Triple Quadrupole Mass Spectrometry             Meher A. Saleem, Anna Mueller, and Sanjoy K. Bhattacharya   10. High-Performance Chromatographic Separation of Cerebrosides             Renaud Sicard and Ralf Landgraf   11. Quantification of Endocannabinoids from Biological Samples Using Liquid Chromatography Tandem Mass Spectrometry             Zhiying Wang, Chenglin Mo, Lynda Bonewald, and Marco Brotto   12. Analysis of Lipids, Fatty Acid, and Cholesterol in Membrane Microdomains             Martin-Paul Agbaga, Mark E. McClellan, and Michael H. Elliott   13. Analysis of Cholesterol Lipids Using Gas Chromatography Mass Spectrometry             Emily J. Neag, Vanessa Collao, and Sanjoy K. Bhattacharya   14. Analyses and Localization of Phosphatidylcholine and Phosphatidylserine in Murine Ocular Tissue Using Imaging Mass Spectrometry             Varun Krishnan, Sean D. Meehan, Colin Hayter, and Sanjoy K. Bhattacharya   15. A Robust Phenotypic Screening Assay Utilizing Human Podocytes to Identify Agents that Modulate Lipid Droplets             Margaret Gurumani, Shamroop Mallela, Javier Varona, Sandra Merscher, Alessia Fornoni, and Hassan Al-Ali   16. Capillary Electrophoresis Plasma Fractionation for Lipids Analysis             Anddre Osmar Valdivia, Carolyn Cray, and Sanjoy K. Bhattacharya   17. Combined Use of MALDI-TOF Mass Spectrometry and 31P NMR Spectroscopy for the Analysis of (Phospho)Lipids             Jenny Leopold, Kathrin M. Engel, Patricia Prabutzki, and Jürgen Schiller   18. Single Step Capture and Targeted Metabolomics of Alkyl-Quinolones in Outer Membrane Vesicles of Pseudomonas aeruginosa             Pallavi Lahiri, Priyakshi Gogoi, and Dipankar Ghosh   19. Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells             Evan B. Stubbs, Jr.   20. Angiogenesis Model of Cornea to Understand the Role of Sphingosine 1-Phosphate             Joseph L. Wilkerson, Sandip K. Basu, and Nawajes Mandal   21. Lipid Extraction and Sample Preservation Techniques for Stable Isotope Analysis and Ecological Assays             Kyle H. Elliott, James D. Roth, Kevin Crook, and David Yurkowski   22. Rapid Analysis of Plasmalogen Individual Species by High-Resolution Mass Spectrometry             Glenda Vasku, Niyazi Acar, and Olivier Berdeaux   23. Untargeted Analysis of Lipids Containing Very Long Chain Fatty Acids in Retina and Retinal Tight Junctions             Julia V. Busik, Hannah Decot, Anna B. Lin, and Todd A. Lydic   24. Analysis of Lipid Contents in Human Trabecular Meshwork Cells by Multiple Reaction Monitoring (MRM) Profiling Lipidomics             Ting Wang and Padmanabhan Paranji Pattabiraman   25. Mass Spectrometry Approaches for Detection and Determination of Prostaglandins from Biological Samples             Zhiying Wang, Chenglin Mo, Kamal Awad, Lynda Bonewald, and Marco Brotto   26. Downloading and Analysis of Metabolomic and Lipidomic Data from Metabolomics Workbench Using MetaboAnalyst 5.0             Ashley Howell and Charles Yaros   27. Accurate Analysis of Lipid Building Blocks Using the Tool LipidOne             Roberto Maria Pellegrino, Matteo Giulietti, Husam B.R. Alabed, Anna Aurora Taddei, Sandra Buratta, Lorena Urbanelli, Francesco Piva, and Carla Emiliani   28. Image Based Longitudinal Characterization of Corneal Wound to Understand the Role of Sphingosine-1-Phosphate             Sandip K. Basu and Nawajes Mandal   29. Characterization of Trabecular Meshwork Mechanical Property Modulation after Application of Lipids             Wyndham Bachelor, Jennifer Arcuri, Sanjoy K. Bhattacharya, and Noël M. Ziebarth   30. C-Laurdan: Membrane Order Visualization of HEK293t Cells by Confocal Microscopy             Sean D. Meehan, Colin Hayter, and Sanjoy K. Bhattacharya   31. Sonication-Based Basic Protocol for Liposome Synthesis             Roberto Mendez

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