Overview

Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. This detailed volume covers genome characterization and analysis, transcriptome analysis and miRNA identification/analysis, forward and reverse genetics, molecular markers, as well as transformation strategies used to investigate gene function and many other topics.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and useful, Legume Genomics: Methods and Protocols aims to serve plant molecular biologists, molecular breeders, plant physiologists and biochemists, developmental biologists, and those interested in plant-microbe interactions.

Full Product Details

Author:   Ray J. Rose
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2013 ed.
Volume:   1069
Dimensions:   Width: 17.80cm , Height: 2.30cm , Length: 25.40cm
Weight:   7.611kg
ISBN:  

9781627036122

ISBN 10:   1627036121
Pages:   316
Publication Date:   01 September 2013
Audience:   Professional and scholarly ,  College/higher education ,  Professional & Vocational ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

The Model Legume Genomes.- Fluorescent In Situ Hybridization (FISH) on Pachytene Chromosomes as a Tool for Genome Characterization.- Targeted Mutagenesis for Functional Analysis of Gene Duplication in Legumes.- RNA-Seq for Transcriptome Analysis in Non-Model Plants.- Functional Analysis of Legume Genome Arrays.- Genome-Wide Identification of MicroRNAs in Medicago truncatula by High-Throughput Sequencing.- Determining Abundance of MicroRNAs and Other Small RNAs in Legumes.- Forward Genetics Screening of Medicago truncatula Tnt1 Insertion Lines.- Reverse Genetics in Medicago truncatula Using a TILLING Mutant Collection.- High-Throughput and Targeted Genotyping of Lotus japonicus LORE1 Insertion Mutants.- Isolation and Forward Genetic Analysis of Developmental Genes in Pea.- Gene Silencing in Medicago truncatula Roots Using RNAi.- Molecular Markers for Genetics and Plant Breeding: The MFLP Marker System and Its Application in Narrow-Leafed Lupin (Lupinus angustifolius).- Stable Transformation of Medicago truncatula cv. Jemalong for Gene Analysis Using Agrobacterium tumefaciens.- Transient Gene Expression in Medicago truncatula Leaves Via Agroinfiltration.- Subcellular Localization of Transiently-Expressed Fluorescent Fusion Proteins.- Proteomics and the Analysis of Nodulation.- Phosphoproteomic Analysis of Peptides.- Plant Metabolomics: From Experimental Design to Knowledge Extraction.

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