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Author:   Jon Lorsch (Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA) ,  Jon Lorsch (Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Volume:   536
Dimensions:   Width: 15.20cm , Height: 1.50cm , Length: 22.90cm
Weight:   0.390kg
ISBN:  

9780124200708

ISBN 10:   0124200702
Pages:   200
Publication Date:   25 March 2014
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Practical Steady-state Enzyme Kinetics Quantification of Protein Concentration using UV absorbance and Coomassie Dyes Preparation of protein samples for mass spectrometry and N-terminal sequencing Lysis of mammalian and Sf9 cells In Vivo [35 S]-Methionine Incorporation Pulse-chase analysis to measure protein degradation Labeling of a Protein with Fluorophores using Maleimide Derivitization Labeling a protein with fluorophores using NHS ester derivitization Protein Derivitization-Expressed Protein Ligation Protein Biotinylation Purification of His-tagged proteins Affinity purification of a recombinant protein expressed as a fusion with the maltose-binding protein (MBP) tag Immunoaffinity purification of proteins Affinity Purification of Protein Complexes Using TAP Tags Strep-tagged protein purification Proteolytic affinity tag cleavage Affinity Pull-down of Proteins using anti-FLAG M2 Agarose Beads Protein Affinity Purification using Intein/Chitin Binding Protein Tags Purification of GST-tagged proteins

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