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Author:   Jon Lorsch (Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA) ,  Jon Lorsch (Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Volume:   539
Dimensions:   Width: 15.20cm , Height: 1.40cm , Length: 22.90cm
Weight:   0.650kg
ISBN:  

9780124201200

ISBN 10:   0124201202
Pages:   240
Publication Date:   29 April 2014
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Small-scale expression of proteins in E. coli Protein expression-Yeast Recombinant protein expression in baculovirus-infected insect cells Single cell cloning of a stable mammalian cell line In vitro synthesis of proteins in bacterial extracts Preparation of a Saccharomyces cerevisiae cell-free extract for in vitro translation Yeast two-hybrid screen UV cross-linking of interacting RNA and protein in cultured cells Analysis of RNA–protein interactions by cell mixing General protein–protein cross-linking Chromatin immunoprecipitation and multiplex sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematode Caenorhabditis elegans PAR-CLIP (photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation): A step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins Determining the RNA specificity and targets of RNA-binding proteins using a three-hybrid system Dissecting a known RNA–protein interaction using a yeast three-hybrid system Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system

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