Overview

This volume provides a complete overview of kupffer cell biology, from isolation to phenotyping. Chapters guide readers through methods and protocols on phenotyping, including RNA sequencing, Mass cytometry and 3D microscopy, as well as gene manipulation in vivo, and disease models from fatty liver disease to cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.     Authoritative and cutting-edge, Kupffer Cells: Methods and Protocols  aims to ensure successful investigation of this growing field. 

Full Product Details

Author:   Myriam Aouadi ,  Valerio Azzimato
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2020
Volume:   2164
Weight:   0.339kg
ISBN:  

9781071607060

ISBN 10:   1071607065
Pages:   160
Publication Date:   01 July 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Kupffer Cell and Hepatocytes Isolation by Gradient Centrifugation from a Single Mouse Liver.- Kupffer Cell Isolation from Human Biopsies.- Liver Macrophage Isolation by Flow Cytometry Sorting.- Kupffer Cell Protein Isolation and Detection by Western Blot.- Kupffer Cell mRNA Sequencing.- Kupffer Cell and Monocyte-derived Macrophage Identification by Immunofluorescence on FFPE Mouse Liver Sections.- Sample Preparation of Optically Cleared Liver Tissue to Identify Liver Macrophages using 3D Microscopy.- Glucan-Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Kupffer cells in mouse liver.- Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model.- Kupffer Cell Characterization by Mass Cytometry.- Enzymatic Quantification of Liver Lipids after Folch Extraction.- In vivo Investigation of High Fat Diet Induced Hepatic Lipid Dysfunctions.- An Obesogenic Dietary Mouse Model of Nonalcoholic Steatohepatitis.- Generating Hepatocellular Carcinoma in Mice By Expressing N-RasG12V Accompanied with Trp53 Silence Using A Transposon-based System Delivered via Hydrodynamic Ail Vein Injection.- Mouse Model of Alcoholic Steatohepatitis.   

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