Overview

Modern enzymology can no longer rely upon indirect inferences derived by steady state kinetic analysis because these are often misleading. Rather, in the era of 'New Enzymology', reactions at the active sites of enzyme are monitored by direct measurement of individual steps in the sequence to establish the pathway of reactions occurring. These quantitative methods can be directly related to high resolution structural information and define the underlying kinetic and thermodynamic basis for catalysis. The goal of a comprehensive kinetic analysis is to identify each step in the reaction pathway, define the rate and equilibrium constants for each step, identify all conformational and chemical intermediates, identify the rate-limiting steps, and ultimately establish the basis for enzymatic specificity and efficiency. This volume contains chapters contributed by the leading experts in the application of modern kinetic methods to complex problems in the study of catalysis by both proteins and RNA catalysts. Each chapter focuses on a unique system and the novel methods used to obtain definitive data that can be directly applied to structural information, giving the much-needed practical details that are often omitted in the general literature. Readers will benefit greatly from the richness of each chapter in describing what is most important in solving the most difficult problems in modern enzymology.

Full Product Details

Author:   Kenneth Johnson (Roger Williams Centennial Professor of Biochemistry, The University of Texas at Austin and Founder and President of Kin Tek Corporation)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   No.267
Dimensions:   Width: 19.50cm , Height: 2.10cm , Length: 25.40cm
Weight:   0.738kg
ISBN:  

9780198524939

ISBN 10:   0198524935
Pages:   276
Publication Date:   25 September 2003
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Out of Print
Availability:   Out of stock  

Table of Contents

1: Johnson: Introduction to kinetic analysis of enzyme systems 2: Anderson: Detection and characterisation of enzyme intermediates 3: Dhavan, Change and Brenowitz: Kinetic analysis of DNA-Protein Interactions by time-resolved synchrotron X-ray footprinting 4: Bevilacqua et al: Kinetic analysis of ribozyme cleavage 5: Patel, Bandwar and Levin: Transient-state kinetics and computational analysis of transcription initiation 6: Webb: A fluorescent sensor to assay inorganic phosphate 7: Goody: What to do if there is no signal 8: Beckett: The biotin regulatory system-kinetic, themodynamic and structural interrogation of a biological switch 9: Palfey: Time resolved spectral analysis 10: Mariano et al: Kinetic analysis of enzyme mutants for identification of catalytic residues

Reviews

a useful book ... the information gained cannot be easily acquired otherwise. A useful contribution. Doodys Publishers Club

Author Information

Kenneth A. Johnson Roger Williams Centennial Professor of Biochemistry Institute for Cell & Molecular Biology, University of Texas, A4800 MBB 3.122, Austin, TX 78735 512-471-0434 512-471-0435 kajohnson@mail.utexas.edu Roger Williams Centennial Professor of Biochemistry The University of Texas at Austin Recipient of Pfizer Award in Enzyme Chemistry, 1989 Founder and President of KinTek Corporation, manufacturer of stopped-flow and quench-flow instruments for fast reaction kinetics. Editorial Board Member, Journal of Biological Chemistry

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