Overview

This book details protocols on classical methods and very modern ex vivo mammalian developmental kidney model systems. Chapters focus on generation of mosaic  embryonic kidneys, culture of the kidney tissues ex vivo, embryonic kidney progenitor cell, 3Dkidney model cell line based cultures, programming the kidney cell fates in human pluripotent cells, protocols to target the functional players in kidney ontogenesis, 4D and 3D ex vivo imaging, drosphila, and computational approaches to kidney ontogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Kidney Organogenesis: Methods and Protocols aims to ensure successful results in thefurther study of this vital field.

Full Product Details

Author:   Seppo Vainio
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   1926
Weight:   0.723kg
ISBN:  

9781493990207

ISBN 10:   1493990209
Pages:   248
Publication Date:   13 February 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Preface... Table of Contents... Contributing Authors... Part I Generation of Mosaic Embryonic Kidneys and Culture of the Kidney Tissues Ex Vivo 1. Generating Genetic Mosaic Mouse Embryos or Organoids for Studies of Kidney Development Frank Costantini 2 Mouse Ex Vivo Kidney Culture Methods Anneliis Ihermann-Hella and Satu Kuure 3 Serum-Free Organ Culture of the Embryonic Mouse Ureter Filipa M. Lopes and Adrian S. Woolf 4 Experimental Tubulogenesis Induction Model in the Mouse Aleksandra Rak-Raszewska Part II Embryonic Kidney Progenitor Cell and 3D Kidney Model Cell Like Based Cultures 5 Dissociation of Tissues for Single Cell Analysis Andrew S. Potter and S. Steven Potter 6 Long Term Culture of Nephron Progenitor Cells Ex Vivo Aaron C. Brown, Ashwani K. Gupta, and Leif Oxburgh 7 3D Cell Culture Models of Epithelial Tissues Kai Zhang and Aki Manninen Part III Programming the Kidney Cell Fates in Human Pluripotent Cells 8 Generation of Three-Dimensional Nephrons from Mouse and Human Pluripotent Stem Cells Yasuhiro Yoshimura, Atsuhiro Taguchi, and Ryuichi Nishinakamura 9 Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells Krithika Hariharan, Petra Reinke, and Andreas Kurtz 10 Protocol to Generate Ureteric Bud Structures from Human iPS Cells Shin-Ichi Mae, Makoto Ryosaka, and Kenji Osafune Part IV Protocols to Target the Functional Players in Kidney Ontogenesis 11 Investigating Aspects of Renal Physiology and Pharmacology in Organ and Organoid Culture Melanie L. Lawrence, Mona Elhendawi, and Jamie A. Davies 12 Virus as Renal Functional Genetic Tools Abhishek Sharma and Sandhanakrishnan Cattavarayane 13 Gene-Editing in 3D Cultured Nephron Progenitor Cell Lines Zhongwei Li, Toshikazu Araoka, and Juan Carlos Izpisua Belmonte Part V 4D and 3D Ex Vivo Imaging of Embryonic Kidney Decellularization 14 Time-Lapse Technologies and 4D Imaging of Kidney Development Ulla Saarela and Ilya Skovorodkin 15 Decellularizing and Recellularizing Adult Mouse Kidneys Theresa Chow, Jennifer Whiteley, and Ian M. Rogers 16 Optical Projection Tomography Imaging to Study Kidney Organogenesis Renata Prunskaite-Hyyrylainen Part VI Drosophila as a Model to Target Conserved Renal Ontogenesis Genes such as the Teashirt 17 Epithelial Function in the Drosophila Malpighian Tubule: An In Vivo Renal Model Shireen-A. Davies, Pablo Cabrero, Richard Marley, Guillermo Martinez Corrales, Saurav Ghimire, Anthony J. Dornan, and Julian A. T. Dow 18 Analysis of the Teashirt Target Genes in Ureteric Bud Development Laurent Fasano, Irene Sanchez-Martin, and Xavier Caubit Part VII Computational Approaches to Kidney Ontogenesis 19 Methods of Computational Analysis in Kidney Development Pauli Tikka and Franz Schaefer

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