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Author:   Shoba Amarnath
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2020
Volume:   2121
Weight:   0.493kg
ISBN:  

9781071603406

ISBN 10:   107160340
Pages:   242
Publication Date:   09 March 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

Protocols for Innate Lymphoid Cell Phenotypic and Functional Characterization: An Overview.- Protocols for Studying Murine ILC Development.- Identification of Murine Innate Lymphoid Cell Subsets in Barrier Tissues and their Draining Lymph Nodes using Flow Cytometry.- Identification and Functional Characterization of Murine Group 3 Innate Lymphoid Cell (ILC3) Subsets in the Intestinal Tract and Associated Lymphoid Tissues.- Identification of Murine and Human Innate Lymphoid Cells in Frozen Tissue Sections using Immunofluorescence.- Assessing Phosphorylation of STAT Transcription Factors in Mouse Innate Lymphoid Cells.- Determining Co-Receptor Expression and Function in Murine ILC2 through Flow Cytometry Characterization and Co-Culture Techniques.- Protocol for Determining the Effect of Neuroendocrine Hormones on Murine ILC Function.- Role of G-Protein Coupled Receptors in Chemotaxis of Innate Lymphoid Cells.- Group 2 ILC Functional Assays inAllergic Airway Inflammation.- Isolation, Culture, and Induction of Plasticity in ILC2s.- Bacterial Infection Allows for Functional Examination of Adoptively Transferred Mouse Innate Lymphoid Cell Subsets.- Isolation, Characterization, and Culture of Intestinal Intraepithelial Lymphocytes.- Isolation and Characterization of Innate Lymphoid Cells within the Murine Tumor Microenvironment.- Microbiota Analysis using Sequencing by Synthesis: From Library Preparation to Sequencing.- Establishing Human Intestinal Enteroid/Organoid Lines from Preterm Infant and Adult Tissue.- Characterizing Innate Lymphoid Cell Phenotype and Function in Human Inflammatory Bowel Disease.- CRISPR/Cas9-Based Gene Engineering of Human Natural Killer Cells: Protocols for Knock-Out and Read-Outs to Evaluate the Efficacy.

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