Overview

This collection of state-of-the-art techniques encompasses the breadth of neurochemical and molecular neurobiology research, ranging from the isolation of neuronal genes and the study of their expression to the analysis of receptor-ligand interactions, to the characterization of the consequences of receptor activation. The methods include electrophysiological techniques to explore the functional properties of receptors present in the membranes of excitable cells, methods to isolate novel genes central to neurobiological processes, and protocols to perform in situ hybridization histochemistry. Other methods cover measuring changes in gene expression, the rapid identification of gene polymorphisms, and the identification and characterization of second messenger pathways. Together with its companion volumes, In Vivo Neuromethods and Cell Neurobiology Techniques, all three cutting-edge works will prove exceptionally useful to those basic and clinical neuroscientists who want to expand the range of their current research or develop competence in complementary methods.

Full Product Details

Author:   Alan A. Boulton ,  Glen B. Baker ,  Alan N. Bateson
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1999 ed.
Volume:   34
Dimensions:   Width: 15.50cm , Height: 2.20cm , Length: 23.50cm
Weight:   1.400kg
ISBN:  

9780896035096

ISBN 10:   0896035093
Pages:   296
Publication Date:   06 November 1998
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Characterization of Receptors by Radiolabeled Ligand Binding Techniques, Martin Davies and Susan M. J. Dunn. Electrophysiological Studies on Receptors In Vitro, Qi-Ying Liu, Anne E. Schaffner, and Jeffrey L. Barker. Subtractive Hydridization: A Method to Identify Novel Genes Induced During Neuronal Programmed Cell Death, H. Michael Tucker and Steven Estus. Messenger RNA Differential Display: An Unbiased Tool to Uncover Alterations in Gene Expression Underlying Biological Processes, Véronique A.-M. I. Tanay and Alan N. Bateson. Localization of mRNAs in Brain Sections by in situ Hybridization Using Oligonucleotide Probes, Alan N. Bateson. Multiprobe Oligonucleotide Solution Hybridization for the Determination of Relative mRNA Levels, Alan N. Bateson and Véronique A.-M. I. Tanay. Competitive RT-PCR to Quantitate Steady-State mRNA Levels, Dennis R. Grayson and Snezana Ikonomovic. A Novel PCR-RFLP Detection Method Using an Optimized Set of Restriction Enzymes, A. Petronis, A. Timinskas, V. Basile, A. Vincente, A. Janulaitis, and J. L. Kennedy. Western Blot Analysis, D. D. Mousseau and V. L. Raghavendra Rao. The Phosphoinositide Signal Transduction System, Richard S. Jope, Xiaohua Li, and Mary A. Pacheco. Protein Phosphorylation, Gail V. W. Johnson and Penny K. Davis. Immunoprecipitation of Receptors, Paul L. Chazot, Simon Pollard, and F. Anne Stephenson. Index.

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