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Author:   R. L. M. Pierik
Publisher:   Springer
Imprint:   Kluwer Academic Publishers
Edition:   illustrated edition
Weight:   0.770kg
ISBN:  

9789024735303

ISBN 10:   9024735300
Pages:   346
Publication Date:   August 1987
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

1. Preface.- 2. Introduction.- 2.1. Outline.- 2.2. Abbreviations and glossary.- 3. History.- 3.1. Introduction.- 3.2. Developments in the Netherlands and other countries.- 4. Types of cultures.- 5. Laboratory equipment.- 5.1. Supplies.- 5.2. The laminar air-flow cabinet.- 5.3. Sterilization of nutrient media.- 5.3.1. Introduction.- 5.3.2. Autoclaving.- 5.3.3. Sterilization by irradiation.- 5.3.4. Sterilization by filtration.- 5.3.5. Sterilization at school and in the home.- 5.4. Preparation room.- 5.5. Cleaning glassware.- 5.6. Robots in the plant tissue culture laboratory.- 6. Preparation and composition of nutrient media.- 6.1. Introduction.- 6.2. Glassware and plastics.- 6.3. Preparation.- 6.4. Composition.- 6.4.1. Water.- 6.4.2. Agar.- 6.4.3. Sugar.- 6.4.4. Mineral nutrition.- 6.4.5. pH.- 6.4.6. Osmotic potential.- 6.4.7. Regulators.- 6.4.7.1. Introduction.- 6.4.7.2. Auxins.- 6.4.7.3. Cytokinins.- 6.4.7.4. Gibberellins.- 6.4.7.5. Other regulators.- 6.4.8. Vitamins.- 6.4.9. Miscellaneous.- 6.5. Commercially prepared media.- 6.6. Storage of nutrient media.- 7. Closure of test tubes and flasks.- 8. Care of plant material.- 9. Sterilization of plant material.- 9.1. Introduction.- 9.2. Chemical sterilization.- 9.3. Apparently sterile cultures.- 9.4. Internal infections.- 9.5. Symbiotic cultures.- 10. Isolation, inoculation and subculturing.- 10.1. Introduction.- 10.2. Isolation.- 10.3. Inoculation.- 10.4. Subculturing.- 11. Mechanization.- 12. The influence of plant material on growth and development.- 13. The influence of physical factors on growth and development.- 13.1. The culture room.- 13.2. Discussion of special physical factors.- 14. The transfer from nutrient medium to soil.- 15. Aids to study.- 15.1. Literature study.- 15.2. Societies and associations.- 15.3. Laboratory notebook, photographs and slides.- 16. Embryo culture.- 16.1. Introduction.- 16.2. Techniques.- 16.3. Factors affecting the success of embryo culture.- 16.4. Practical applications.- 17. Germination of orchid seeds.- 17.1. Introduction.- 17.2. Factors affecting germination and growth.- 18. Vegetative propagation of orchids.- 18.1. Introduction.- 18.2. Meristem culture.- 18.2.1. Production of virus-free plants.- 18.2.2. Propagation through meristem culture.- 18.3. Other methods of propagation.- 18.4. Variation arising during culture.- 18.5. Practical applications.- 19. Production of disease-free plants.- 19.1. Introduction.- 19.2. Production of virus-free plants.- 19.2.1. Heat treatment.- 19.2.2. Meristem culture.- 19.2.2.1. History.- 19.2.2.2. Accomplishment.- 19.2.3. Heat treatment and meristem culture.- 19.2.4. Adventitious shoot formation, eventually followed by meristem culture.- 19.2.5. Virus-free plants produced from callus and protoplasts.- 19.2.6. Grafting of meristems on virus-free (seedling) rootstocks (micrografting).- 19.2.7. Virus identification.- 19.3. Production of bacteria- and fungal-free plants by meristem culture.- 20. Vegetative propagation.- 20.1. General introduction.- 20.2. Single-node culture.- 20.3. The axillary bud method.- 20.4. Regeneration of explants.- 20.4.1. Introduction.- 20.4.2. Adventitious root formation.- 20.4.3. Adventitious shoot formation.- 20.5. Callus induction, callus culture and regeneration of organs and embryos.- 20.5.1. Introduction.- 20.5.2. Callus induction.- 20.5.3. Callus culture.- 20.5.3.1. Introduction.- 20.5.3.2. On solid media.- 20.5.3.3. In liquid media.- 20.5.4. Regeneration of organs and embryos.- 20.5.4.1. Introduction.- 20.5.4.2. Regeneration of organs.- 20.5.4.3. Regeneration of embryos.- 20.5.4.4. Nucellar polyembryony.- 20.6. Regeneration of plants from single cells.- 20.7. Synthetic seeds.- 21. Somaclonal variation.- 22. Test tube fertilization.- 23. Production of haploids.- 23.1. General introduction.- 23.2. Obtaining haploids in vitro.- 23.3. Problems associated with haploid induction.- 24. Genetic manipulation.- 24.1. General introduction.- 24.2. Description.- 24.3. Prerequisites for the use of genetic manipulation.- 24.4. Somatic hybridization.- 24.4.1. Outline.- 24.4.2. Discussion of the different phases involved.- 24.5. The relevance of somatic hybridization.- 24.6. Disadvantages and problems of somatic hybridization.- 24.7. Selection procedures after somatic hybridization.- 24.8. Selection of mutants.- 24.9. Transformation by Agrobacterium tumefaciens.- 25. Miscellaneous applications.- 25.1. In phytopathology.- 25.1.1. Outline.- 25.1.2. Transport of disease-free plant material.- 25.2. In plant breeding.- 25.2.1. The development of chimeras in vitro.- 25.2.2. Separation of chimeras and isolation of mutants.- 25.2.3. Obtaining tetraploids and triploids; induction of chromosome loss.- 25.2.4. Mutation induction in vitro.- 25.2.5. Storage of plant material in vitro.- 25.3. The biosynthesis of substances in vitro.- 26. In vitro cloning of plants in the Netherlands.- 27. Index.- 28. Literature cited.

Reviews

'... illustrations of high quality ... a good index and an up-to-date bibliography. Overall, the book succeeds in the authors' stated aim of providing an introductory text on in vitro culture of higher plants for university students as well as serving as a practical handbook for technicians and research assistants. There is also much to interest the more senior research worker. This book should be much in demand. ' Commonwealth Agricultural Bureau

... illustrations of high quality ... a good index and an up-to-date bibliography. Overall, the book succeeds in the authors' stated aim of providing an introductory text on in vitro culture of higher plants for university students as well as serving as a practical handbook for technicians and research assistants. There is also much to interest the more senior research worker. This book should be much in demand.' Commonwealth Agricultural Bureau

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