Overview

In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.

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9780896039520

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Reviews

Investigators working in the general area of DNA damage, including apoptosis, will find this book of use. The protocols are sufficiently detailed so as to allow experienced laboratory workers access to the described methods...the broad spectrum of methods described allows for individual investigators to exercise options. - Doody's Health Sciences Book Review Journal Here, a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). European Journal of Histochemistry

Investigators working in the general area of DNA damage, including apoptosis, will find this book of use. The protocols are sufficiently detailed so as to allow experienced laboratory workers access to the described methods...the broad spectrum of methods described allows for individual investigators to exercise options. -Doody's Health Sciences Book Review Journal Here, a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). - European Journal of Histochemistry

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