Overview

A major new series of methods books for the life science community, providing authoritative sources of research information and detailed practical guidance with references to the primary literature that are graded to guide readers to the most useful articles. Rather than simply offering a selection of methods from each authors' own lab, Methods Express volumes critically assess the available techniques and approaches and advise on the suitability of each for different experimental goals. Specific protocols are then recommended and are provided in full practical detail. All authors provide detailed advice on troubleshooting problems, and example data generated by the recommended techniques are also given as benchmarks. Immunohistochemistry: Methods Express covers all the latest techniques and approaches, including: choice of antibodies; selection of reporter labels; staining techniques with single and multiple labels; use of confocal microscopy and tissue microarrays; image capture; analysis and quantification; troubleshooting and quality assurance; automation. This book will be welcomed both by experienced researchers wishing to take advantage of the latest developments and by newcomers to the field who need to rapidly identify reliable techniques and approaches that will yield success in the laboratory.

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9781904842170

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