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OverviewFull Product DetailsAuthor: James Gosling (Department of Biochemistry, Department of Biochemistry, National University of Ireland)Publisher: Oxford University Press Imprint: Oxford University Press Volume: 228 Dimensions: Width: 18.90cm , Height: 1.80cm , Length: 24.50cm Weight: 0.714kg ISBN: 9780199637102ISBN 10: 0199637105 Pages: 324 Publication Date: 15 June 2000 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: To order ![]() Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us. Table of ContentsAnalysis by specific binding ; Raising antibodies ; Choosing and characterizing antibodies ; Labels and endpoints ; Solid-phase reagents ; Standard and immunogens ; Optimization ; Validation ; Data processing ; Quality assuranceReviews<br> It is indeed an excellent compilation of the basic steps in immunoassay design and development. Dr. Gosling has assembled an accomplished group of immunoassay experts who provide clear and uncomplicated accounts of the practical aspects of immunoassays. A useful feature of the book is the inclusion of easily identified, step-by-step protocols and tips throughout the text. This book will be an invaluable practical resource for students and research workers trying to come to grips with the complexities of immunoassay technology. -- Larry J. Kricka, Clinical Chemistry, 47, No. 3, 2001<p><br> There are now so many variations on the use of the core technology of the immunoassay, the antigen-antibody reaction, that when I opened this book I was a little sceptical as to whether James Gosling would be able to achieve the goals set for the Practical Approach series. ... [A]ny text that sets out to provide a series of protocols to help the experimental scientist to measure a specific analyte must ensure that there is guidance on method design, optimization, calibration and validation--that is, to keep the feet on the ground! The book achieves this objective and includes useful tips and contact names and, although the latter will go out of date, it adds value. ... This is a useful book for the analyst who needs to develop and use an immunoassay. It is also a useful text for the analyst who uses a 'commercial assay' and who has not had any part in its development, because it provides the background knowledge that will help with troubleshooting. --Trends in Biochemical Sciences<p><br> It is indeed an excellent compilation of the basic steps in immunoassay design and development. Dr. Gosling has assembled an accomplished group of immunoassay experts who provide clear and uncomplicated accounts of the practical aspects of immunoassays. A useful feature of the book is the inclusion of easily identified, step-by-step protocols and tips throughout the text. This book will be an invaluable practical resource for students and research workers trying to come to grips with the complexities of immunoassay technology. -- Larry J. Kricka, Clinical Chemistry, 47, No. 3, 2001<br> There are now so many variations on the use of the core technology of the immunoassay, the antigen-antibody reaction, that when I opened this book I was a little sceptical as to whether James Gosling would be able to achieve the goals set for the Practical Approach series. ... [A]ny text that sets out to provide a series of protocols to help the experimental scientist to measure a specific analyte must ensure that there is guidance on method design, optimization, calibration and validation--that is, to keep the feet on the ground! The book achieves this objective and includes useful tips and contact names and, although the latter will go out of date, it adds value. ... This is a useful book for the analyst who needs to develop and use an immunoassay. It is also a useful text for the analyst who uses a 'commercial assay' and who has not had any part in its development, because it provides the background knowledge that will help with troubleshooting. --Trends in Biochemical Sciences<br> Author InformationTab Content 6Author Website:Countries AvailableAll regions |