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This dissertation, Identification of Tumor-associated Proteins in Human Prostatic Epithelial Cell Lines & Squamous Cell Carcinoma of Head and Neck by Proteomic Technology by Jia, Chen, 陳珈, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled Identification of Tumor-Associated Proteins in Human Prostatic Epithelial Cell Lines & Squamous Cell Carcinoma of Head and Neck by Proteomic Technology Submitted by CHEN JIA for the degree of Master of Philosophy at The University of Hong Kong in October 2004 Proteomics defines a research field aiming to characterize molecular and cellular dynamics in protein expression and function on a global level. It has been a powerful tool widely used in recent years in protein expression mapping by two-dimentioanal electrophoresis and mass spectrometry. Prostate cancer is the most commonly diagnosed cancer in Western men. The incidence and mortality rates of prostate cancer in Asian countries, such as China and Japan, appear to increase much in the last two decades. We use proteomics to examine four HPE cell models: 267B1, Ki-ros/267Bl, Ki-ros/267Bl/m and XR/267B1, representing immortalized and ras/XR transformed/tumorigenic HPE cells. Not too many proteins were identified as differently expressed in the transformed/tumorigenic HPE cell lines and the immortalized HPE cells except for the fragments of 7 proteinsincluding GRP78 (up-regulated in ras transformed/tumorigenic HPE cell lines only) and HSP70 (up-regulated in both ras transformed/tumorigenic and XR transformed HPE cell line) from HSP70 family, nuclear splicing related hnRNPs (hnRNP Al and A2) and snRNP (SF3a60) and energy metabolism related DLST and ATP synthase p chain (all these 5 were up-regualted in both ras and XR transformed not in ras tumorigenic HPE cell lines). Further investigation is needed to characterize the proteome changes in these HPE cell models to fully understand their relationship, the impact of ras oncogene transformation and the X-ray irradiation transformation, and also the tumorigenic process occurring on the tissue cultured re-established HPE tumor cells. Squamous cell carcinoma (SCC) of the head and neck is the fifth most common cancer worldwide and is closely related to the practice of tobacco smoking and betel squid chewing. The high recurrence and low survival rates of HNSCC require our continued efforts to understand the pathogenesis of the disease for designing better therapeutic strategies. Proteomic technology has also been used in our studies to identify biomarkers for squamous cell carcinomas of tongue and buccal mucoca with the subject specimens of raw tumor tissue, and surrounding mucosa is used as control. In both studies, significant and consistent variations were found in between the tumor tissue and the control. In tongue SCC proteomic analysis, a number of tumor-associated proteins, including HSP60, HSP27, Cryst-B, ATP synthase P, calgranulin B, myosin, tropomyosin and galectin 1, were consistently found to be significantly altered in their expression levels in the tongue carcinoma tissues, compared with their paired normal mucosae. The expression profile portrays a global protein alteration that appears specific to oral tongue cancer. In buccal SCC analysis, 23 proteins or protein in fragments were found to have elevated expressionlevels in the SCC tissues compared to the normal mucosa except that Cryst-B was down-regulated. Proteins of the increased expression include glycolytic enzymes, HSPs, tumor antigens, cytoskeleton proteins, enzymes involved in detoxification and anti-oxidation systems, and protei

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