Human Embryonic Stem Cell Protocols

Author:   Kursad Turksen
Publisher:   Humana Press Inc.
Edition:   3rd ed. 2015
Volume:   1307
ISBN:  

9781493926671


Pages:   399
Publication Date:   11 August 2015
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

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Human Embryonic Stem Cell Protocols


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Author:   Kursad Turksen
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   3rd ed. 2015
Volume:   1307
Dimensions:   Width: 17.80cm , Height: 2.80cm , Length: 25.40cm
Weight:   1.294kg
ISBN:  

9781493926671


ISBN 10:   1493926675
Pages:   399
Publication Date:   11 August 2015
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Blastocysts.- Characterizing Pluripotent Stem Cells Using the TaqMan® hPSC ScorecardTM Panel.- Growth of Human Pluripotent Stem Cells Using Functional Human Extracellular Matrix.- Efficient Expansion of Dissociated Human Pluripotent Stem Cells Using a Synthetic Substrate.- Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryonic Stem Cells.- Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR.- Study of Gap Junctions in Human Embryonic Stem Cells.- Immunofluorescence Microscopy and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free Conditions.- Analysis of Intracellular Calcium Signaling in Human Embryonic Stem Cells.- Genetic Manipulation of Human Embryonic Stem Cells.- Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 System.- Use of Multicolor Flow Cytometry for Isolation of Specific Cell Populations Deriving from Differentiated Human Embryonic Stem Cells.- Definitive Endoderm Differentiation of Human Embryonic Stem Cells Combined with Selective Elimination of Undifferentiated Cells by Methionine Deprivation.- Derivation of Endothelial Cells and Pericytes from Human Pluripotent Stem Cells.- Differentiation of Human Embryonic Stem Cells on Periodontal Ligament Fibroblasts.- Derivation of Epithelial Cells from Human Embryonic Stem Cells as an In Vitro Model of Vocal Mucosa.- Human Embryonic and Hepatic Stem Cell Differentiation Visualized in Two and Three Dimensions Based on Serial Sections.- Derivation of Chondrogenic Cells from Human Embryonic Stem Cells for Cartilage Tissue Engineering.- Microgrooved Surface Modulates Neuron Differentiation in Human Embryonic Stem Cells.- Directed Differentiation of HumanEmbryonic Stem Cells into Neural Progenitors.- Direct Conversion of Pluripotent Human Embryonic Stem Cells Under Defined Culture Conditions into Human Neuronal or Cardiomyocyte Cell Therapy Derivatives.- Generation of Epithelial Cell Populations from Human Pluripotent Stem Cells Using a Small Molecule Inhibitor of Src Family Kinases.- Dual-SMAD Inhibition/WNT Activation-Based Methods to Induce Neural Crest and Derivatives from Human Pluripotent Stem Cells.- Accelerated Three-Dimensional Neuroepithelium Formation from Human Embryonic Stem Cells and Its Use for Quantitative Differentiation to Human Retinal Pigment Epithelium.- Efficient Production of Photoreceptor Precursor Cells from Human Embryonic Stem Cells.- Generation of Megakaryocytes and Platelets from Human Pluripotent Stem Cells.- A Simple Protocol for the Generation of Cardiomyocytes from Human Pluripotent Stem Cells.

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