Overview

This new edition explores and provides an update on the biology and pathogenesis of human cytomegalovirus infection.  Modern techniques that are currently being utilized to investigate the molecular aspects of viral infection, as well as how these new research studies are leading to new approaches to mitigate disease, are also provided. Given the key role the virus plays in significant acute and chronic human disease in all stages of life, from newborns to seniors, the need for clear methodologies to further explore the biology of HCMV infection and mitigation strategies is readily apparent. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Human Cytomegaloviruses: Methods and Protocols, Second Edition serves as an ideal scientific reference for basic and clinical scientists and medical personnel on the modern understanding of the pathobiology of the virus, and the approaches, techniques, and models to study human cytomegalovirus infection and disease.

Full Product Details

Author:   Andrew D. Yurochko
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2021
Volume:   2244
Weight:   0.905kg
ISBN:  

9781071611135

ISBN 10:   1071611135
Pages:   468
Publication Date:   09 February 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Overview of Human Cytomegalovirus Pathogenesis             Heather L. Fulkerson, Maciej T. Nogalski, Donna Collins-McMillen, and Andrew D. Yurochko   2. Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies             Giada Frascaroli and Christian Sinzger   3. Use of Diploid Human Fibroblasts as a Model System to Culture, Grow, and Study Human Cytomegalovirus Infection             Elizabeth A. Fortunato   4. The Use of Primary Human Cells to Analyze Human Cytomegalovirus Biology             Emma Poole, Ian Groves, Sarah Jackson, Mark Wills, and John Sinclair   5. Human Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation             Megan Peppenelli, Jason Buehler, and Felicia Goodrum   6. Collection and Isolation of CD14+ Primary Human Monocytes via Dual Density Centrifugation as a Model System to Study Human Cytomegalovirus Infection and Pathogenesis             Bailey S. Mosher, Heather Fulkerson, and Andrew D. Yurochko   7. Stable and Inducible Gene Knockdown in Primary Human Fibroblasts: A Versatile Tool to Study the Role of Human Cytomegalovirus Host Cell Factors             Anne-Charlotte Stilp, Patrick König, Myriam Scherer, and Thomas Stamminger   8. Construction of Human Cytomegalovirus Mutants with Markerless BAC Mutagenesis             M. Zeeshan Chaudhry, Martin Messerle, and Luka Cicin-Sain   9. Methods for Studying the Function of Cytomegalovirus GPCRs             Christine M. O’Connor and William E. Miller   10. Analysis of Cytomegalovirus Glycoprotein and Cellular Receptor Interactions             Jamil Mahmud and Gary C. Chan   11. Antibody-Independent Quantification of Cytomegalovirus Virion Protein Incorporation Using HiBiT             Iris K.A. Jones and Daniel N. Streblow   12. Use of a Phosphoproteomic Screen to Profile Early Changes during HCMV Infection of Human Monocytes             Liudmila S. Chesnokova and Andrew D. Yurochko   13. A Generally Applicable CRISPR/Cas9 Screening Technique to Identify Host Genes Required for Virus Infection as Applied to Human Cytomegalovirus (HCMV) Infection of Epithelial Cells             Xiaofei E and Timothy F. Kowalik   14. Quantitative Electron Microscopy to Study HCMV Morphogenesis             Clarissa Read, Paul Walther, and Jens von Einem   15. Detection of Cytomegalovirus Interleukin 10 (cmvIL-10) by Enzyme-Linked Immunosorbent Assay (ELISA)             Vivian P. Young, Margarette C. Mariano, Lionel Faure, and Juliet V. Spencer   16. Techniques for Characterizing Cytomegalovirus-Encoded miRNAs             Nicole L. Diggins, Lindsey B. Crawford, Hillary M. Struthers, Lauren M. Hook, Igor Landais, Rebecca L. Skalsky, and Meaghan H. Hancock   17. Development of a huBLT Mouse Model to Study HCMV Latency, Reactivation, and Immune Response             Lindsey B. Crawford and Patrizia Caposio   18. Rodent Models of Congenital Cytomegalovirus Infection             Berislav Lisnić, Jelena Tomac, Djurdjica Cekinovic, Stipan Jonjic, and Vanda Juranić Lisnić   19. Recent Approaches and Strategies in the Generation of Anti-Human Cytomegalovirus Vaccines             Suresh B. Boppana and William J. Britt

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions