Overview

A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicinea series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Full Product Details

Author:   Joanna Picot
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 2nd ed. 2005
Volume:   107
Dimensions:   Width: 15.20cm , Height: 2.10cm , Length: 22.90cm
Weight:   0.546kg
ISBN:  

9781617374319

ISBN 10:   1617374318
Pages:   360
Publication Date:   10 November 2010
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Establishment and Maintenance of Normal Human Keratinocyte Cultures.- Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters.- Isolation and Culture of Human Osteoblasts.- Human Osteoclast Culture From Peripheral Blood Monocytes.- Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation.- Human Myoblasts and Muscle-Derived SP Cells.- Cell Cultures of Autopsy-Derived Fibroblasts.- Primary Culture and Differentiation of Human Adipocyte Precursor Cells.- Human Mononuclear Phagocytes in Tissue Culture.- Purification of Peripheral Blood Natural Killer Cells.- Human Fetal Brain Cell Culture.- Culturing Human Schwann Cells.- Well-Differentiated Human Airway Epithelial Cell Cultures.- Isolation and Culture of Human Alveolar Epithelial Cells.- A New Approach to Primary Culture of Human Gastric Epithelium.- Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a Noninjurious Approach.- Isolation and Culture of Human Hepatocytes.- Glomerular Epithelial and Mesangial Cell Culture and Characterization.- Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules.- Culture of Parathyroid Cells.- Long-Term Culture and Maintenance of Human Islets of Langerhans in Memphis Serum-Free Media.- Primary Culture of Human Antral Endocrine and Epithelial Cells.- Conjunctiva Organ and Cell Culture.- Establishment, Maintenance, and Transfection of In Vitro Cultures of Human Retinal Pigment Epithelium.

Reviews

This book offers a welcome text which is value to anybody undertaking human cell culture. - Cell Biochemistry and Function ...a very useful general text for researchers involved in human cell cultures. -Immunology News From Reviews of the First Edition... ...packed full of methods for the culture of cells from human tissues...an excellent book full of very useful information. -Trends in Cell Biology ...an invaluable and highly recommended resource for cell culturists setting up new systems. -Molecular Biotechnology ...will be of significance to a very wide range of scientists. -ISSX Newsletter ...has a wealth of down-to-earth, ready-to-use information. -Modern Pathology ..a valuable reference work for workers looking for detailed methodology for culturing the specific cell types covered. -BTS Newsletter

This book offers a welcome text which is value to anybody undertaking human cell culture. - Cell Biochemistry and Function ...a very useful general text for researchers involved in human cell cultures. -Immunology News From Reviews of the First Edition... ...packed full of methods for the culture of cells from human tissues...an excellent book full of very useful information. -Trends in Cell Biology ...an invaluable and highly recommended resource for cell culturists setting up new systems. -Molecular Biotechnology ...will be of significance to a very wide range of scientists. -ISSX Newsletter ...has a wealth of down-to-earth, ready-to-use information. -Modern Pathology ..a valuable reference work for workers looking for detailed methodology for culturing the specific cell types covered. -BTS Newsletter

This book offers a welcome text which is value to anybody undertaking human cell culture. - Cell Biochemistry and Function ...a very useful general text for researchers involved in human cell cultures. -Immunology NewsFrom Reviews of the First Edition... ...packed full of methods for the culture of cells from human tissues...an excellent book full of very useful information. -Trends in Cell Biology ...an invaluable and highly recommended resource for cell culturists setting up new systems. -Molecular Biotechnology ...will be of significance to a very wide range of scientists. -ISSX Newsletter ...has a wealth of down-to-earth, ready-to-use information. -Modern Pathology ..a valuable reference work for workers looking for detailed methodology for culturing the specific cell types covered. -BTS Newsletter

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