Overview

This fully updated volume serves as multidisciplinary compendium of approaches and techniques employed to analyze the role of different molecules, processes, or strategies used by different guests to survive and proliferate in their associations with eukaryotic hosts. Beginning with animal-pathogen interactions, the book then continues with chapters exploring virus-host interactions, plant-microbe interactions, as well as different molecular techniques that were initially applied to non-pathogenic interactions but can be adapted to study other host-pathogen associations. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Host-Pathogen Interactions: Methods and Protocols, Second Edition contributes to the study of host-pathogen interactions with numerous techniques that can be used in a variety of bacteria.

Full Product Details

Author:   Carlos Medina ,  Francisco Javier López-Baena
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2024
Volume:   2751
Weight:   0.736kg
ISBN:  

9781071636169

ISBN 10:   1071636162
Pages:   270
Publication Date:   25 January 2024
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Introduction   1. Host-Pathogen Interaction: Biology and Public Health             Richard Ponce-Cusi, Leny Bravo, Kevin J. Paez, Joseph A. Pinto, and Nesstor Pilco-Ferreto   2. Genetic Association Studies in Host-Pathogen Interaction Analysis             Marina Laplana, José Luis Royo, and Luis Miguel Real   Part II: Viral-Host Interactions   3. Live-Cell Fluorescence Imaging for Virus/Host Interactions             Francesca J. Scribano, Kristen A. Engevik, J. Thomas Gebert, and Joseph M. Hyser   4. Construction of a Mycoviral Infectious Clone for Reverse Genetics in Botrytis cinerea             Laura Córdoba, Ana Ruiz-Padilla, Javier Pardo-Medina, Julio L. Rodríguez-Romero, and María A. Ayllón   Part III: Plant-Pathogens Interactions   5.  Exopolysaccharide Production and Precipitation Method as a Tool to Study Virulence Factors             Natalia Mielnichuk, Constanza M. Joya, María A. Monachesi, and Romina P. Bertani   6. Virulence-Related Assays for Investigation of the Acidovorax citrulli-Cucurbitaceae Pathosystem             Francisco Pérez-Montaño, Irene Jiménez-Guerrero, Dafna Tamir-Ariel, and Saul Burdman   7. Dual-Fluorescence Chromosome-Located Labeling System for Accurate In Vivo Single-Cell Gene Expression Analysis in Pseudomonas syringae             Nieves López-Pagán, José S. Rufián, Javier Ruiz-Albert, and Carmen R. Beuzón   8. A Robust Method to Perform In Vitro and In Planta Interbacterial Competition Assays: Killing Plant Pathogens by a Potent Biocontrol Agent             Cristina Civantos, Adrián Ruiz, and Patricia Bernal   Part IV: Molecular Methods for Plant-Interacting Bacteria   9. Quantification of Mixed-Linkage β-Glucan (MLG) in Bacteria             Juan Antonio Marchante, Lucía Ruíz-Sáez, Socorro Muñoz, Juan Sanjuán, and Daniel Pérez-Mendoza   10. Surface Plasmon Resonance as a Tool to Elucidate the Molecular Determinants of Key Transcriptional Regulators Controlling Rhizobial Lifestyles             Laura Tomás-Gallardo, Juan J. Cabrera, and Socorro Mesa   11. Microscope Subcellular Localization of Plant-Interacting Bacterial Effectors in Animal Cell Cultures             Irene Jiménez-Guerrero, Francisco Javier López-Baena, and Carlos Medina   12. A Workflow for the Functional Characterization of Noncoding RNAs in Legume Symbiotic Bacteria             Natalia I. García-Tomsig, Sabina K. Guedes-García, and José I. Jiménez-Zurdo   13. Methods for Studying Swimming and Surface Motilities in Rhizobia             Francisco Fuentes-Romero, Cynthia Alías-Villegas, Pilar Navarro-Gómez, Sebastián Acosta-Jurado, Lydia M. Bernabéu-Roda, Virginia Cuéllar, María J. Soto, and José M. Vinardell   14. Isolation, Quantification, and Visualization of Extracellular Membrane Vesicles in Rhizobia Under Free-Living Conditions             Paula Ayala-García, Natalia Moreno-de Castro, Irene Jiménez-Guerrero, Mathias Müsken, Alejandro Arce-Rodríguez, Francisco Pérez-Montaño, and José Manuel Borrero-de Acuña   15. Isolation of Rhizobial Extracellular Membrane Vesicles from Bacteroids             Paula Ayala-García, Irene Jiménez-Guerrero, Mathias Müsken, Francisco Javier Ollero, José Manuel Borrero-De Acuña, and Francisco Pérez-Montaño   16. Nod Factor Lipopolysaccharide Purification to Study Nitrogen-Fixing Bacteria Symbiosis with Legumes             Catherine Jacott, Sara Lozano-Morillo, and Pablo del Cerro   17. A Novel System to Selective Tagging of Sinorhizobium fredii Symbiotic Plasmids             Ana María Cutiño, María del Carmen Sánchez-Aguilar, José Enrique Ruiz-Sáinz, María del Rosario Espuny, Francisco Javier Ollero, and Carlos Medina   18. New Inoculation Strategy for Legume Based on Rhizobium-Metabolite Co-Encapsulation             Adriana B. Cesari, Verónica E. Castilla Marín, Luciana Nieva Muratore, Natalia S. Paulucci, and Marta S. Dardanelli

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