Overview

This book systematically illustrates theories and technologies in Histochemistry, including different kinds of enzymes, immunohistochemistry, polymerase chain reaction, related electron microscopic cytochemical techniques as well as the quantitative assay metrology. Abundant experiments as well as vivid images are demonstrated, making the book an essential reference for both graduate students and researchers in biochemistry.

Full Product Details

Author:   Jinsong Zhou ,  Xi'an Jiaotong University Press Co.
Publisher:   De Gruyter
Imprint:   De Gruyter
Dimensions:   Width: 17.00cm , Height: 1.50cm , Length: 24.00cm
Weight:   0.484kg
ISBN:  

9783110524826

ISBN 10:   3110524821
Pages:   242
Publication Date:   21 August 2017
Audience:   Professional and scholarly ,  Professional and scholarly ,  Professional & Vocational ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Available To Order  
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Table of Contents

Contents Chapter 1 Introduction 1.1 The contents and theories in Histochemistry 1.2 The basic requirements of Histochemistry methods Chapter 2 The Tissue Preparation 2.1 Tissue collection 2.2 Fixation 2.3 Tissue washing-up, dehydration and clearance 2.4 Embedding 2.5 Sectioning 2.6 Adherence and mounting 2.7 Buffer Chapter 3 The Carbohydrate and its Derivatives Histochemistry 3.1 Classification 3.2 Histochemistry methods Chapter 4 The Nucleic Acid Histochemistry 4.1 DNA histochemical demonstration 4.2 Comparison demonstration of DNA and RNA Histochmistry Chapter 5 The Lipid Histochemistry 5.1 Overview 5.2 Demonstration of lipids with physical methods 5.3 Demonstration of lipids with chemical methods Chapter 6 Enzyme Histochemistry 6.1 Enzyme and its basic histochemical theory 6.2 Histochemistry for common enzymes Chapter 7 The Basic Theory of Immunohistochemistry 7.1 Basic Immunology 7.2 Common markers and their detection 7.3 Basic conditions Chapter 8 Common Methods Used in Immunohistochemistry 8.1 Principles 8.2 Immunofluorescence method 8.3 Immunoenzyme technique 8.4 Avidin-biotin method 8.5 Protein A method 8.6 Immunogold method and immune gold-silver method Chapter 9 Specificity and Sensitivity of Immunohistochemistry 9.1 Specificity and specific staining 9.2 Control experiment 9.3 The methods to enhance immunohistochemical sensitivity Chapter 10 The Double-Staining in Immunohistochemistry 10.1 Double-staining Immunohistochemistry on serial section 10.2 Immunofluorescence double labelling technique 10.3 Immunoenzyme double staining technique 10.4 Immunoenzyme-Immunofluorescence double staining 10.5 Immunoenzyme-Immunogold double staining Chapter 11 The Lectin Histochemistry 11.1 Characteristics and application of lectin 11.2 Application of lectin Histochemistry Chapter 12 The progress of in Situ Display 12.1 The envision method 12.2 The catalysed signal amplification method 12.3 The ferric oxide alternative method for HRP Chapter 13 In Situ Hybridization Immunohistochemistry 13.1 Basic principle 13.2 Probe preparation 13.3 Procedure of in situ hybridization histochemistry 13.4 Factors affecting in situ hybridization 13.5 Control test Chapter 14 In Situ Polymerase Chain Reaction Technique 14.1 Basic principle 14.2 Basic types 14.3 Procedure 14.4 Application of in situ PCR technology Chapter 15 Electron Microscopic Cytochemical Technique 15.1 Electron microscopic enzyme cytochemistry technology 15.2 Electron microscopic Immunocytochemical technique Chapter 16 The Quantitative Assay of Histochemistry Results 16.1 The photomicrography 16.2 The image analyzer 16.3 The flow cytometry 16.4 The laser scanning confocal microscopy References

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Author Information

Jinsong Zhou, Xi’an Jiaotong University, Shannxi Province, China

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