Overview

This book provides a comprehensive account of the principles and applications of affinity or highly selective separations in biotechnology, including the use of membranes and phase boundaries. Each chapter covers the fundamental chemistry and biochemistry, before describing industrial applications and developments. A team of internationally respected contributors provides an up-to-date perspective on this field of considerable commercial importance.

Full Product Details

Author:   G. Street
Publisher:   Chapman and Hall
Imprint:   Chapman and Hall
Edition:   1994 ed.
Dimensions:   Width: 15.50cm , Height: 1.70cm , Length: 23.50cm
Weight:   1.160kg
ISBN:  

9780751400519

ISBN 10:   0751400513
Pages:   231
Publication Date:   31 August 1994
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

1 Overview.- References.- 2 Affinity precipitation.- 2.1 Introduction.- 2.2 Various formats of affinity precipitation.- 2.3 Heterobifunctional ligands.- 2.4 Use of macro affinity ligands.- 2.5 Practical applications of affinity precipitation.- 2.6 Affinity precipitation by heterobifunctional ligands.- 2.7 Comparison of affinity precipitation with other affinity techniques.- 2.8 Conclusions.- References.- 3 Membrane-based affinity separation processes.- 3.1 Introduction.- 3.2 Chemical and physical feature of the membrane matrix.- 3.3 Purification protocol.- 3.4 Ligand coupling.- 3.5. Stability of active membranes.- 3.6 Reuse of ligand coupled membranes.- 3.7 Storage of ligand coupled membranes in buffer.- 3.8 Ligand leaching.- 3.9 Efficiency of ligand coupling.- 3.10 Effect of flow rates on affinity purification of IgG.- 3.11 Exhaustive purification of antibodies.- 3.12 Effect of sample recirculation on yields.- 3.13 Purification of IgG from ascites fluid and serum.- 3.14 Efficiency in product recovery.- 3.15 Applications.- 3.16 Scale-up.- 3.17 Discussion.- References.- 4 Affinity partitioning.- 4.1 Introduction.- 4.2 Two-phase systems.- 4.3 General ways of steering the partitioning.- 4.4 Affinity partitioning.- 4.5 Large-scale extractions.- 4.6 Use of two-phase systems in bioreactors.- 4.7 Non-protein partitioning.- 4.8 Alternative types of affinity ligands.- 4.9 Counter-current distribution.- 4.10 Conclusions.- References.- 5 The use of reverse micelles for the separation of proteins.- 5.1 Introduction.- 5.2 Description of reverse micelles.- 5.3 The reverse micellar extraction method.- 5.4 Protein distribution between an aqueous and a conjugated reverse micellar phase.- 5.5 Mass transfer of protein extraction.- 5.6 Process development.- 5.7 Examples of reverse micellarapplications for protein separation.- 5.8 Conclusions.- References.- 6 The chemistry and engineering of affinity chromatography.- 6.1 Introduction.- 6.2 The role of AC in protein purification.- 6.3 Affinity packings.- 6.4 Characterization of AC packings.- 6.5 Modelling and design of affinity chromatography columns.- 6.6 Notation.- References.- 7 Protein fusions as an aid to purification.- 7.1 Introduction.- 7.2 Choice of host organism.- 7.3 Induction of expression.- 7.4 Solubilisation of recombinant proteins.- 7.5 Vectors.- 7.6 Affinity purification.- 7.7 Thrombin cleavage of fusion proteins.- 7.8 General discussion.- References.- 8 Chiral separations.- 8.1 Introduction.- 8.2 Some stereochemical terms.- 8.3 Chirality and biological systems.- 8.4 Methods available for chiral separation.- 8.5 Conclusion.- References.- 9 Molecular imprinting—a versatile technique for the preparation of separation materials of predetermined selectivity.- 9.1 Introduction.- 9.2 Preparation of molecular imprints.- 9.3 Recognition in molecularly imprinted polymers.- 9.4 Application.- 9.5 Conclusions.- References.

Reviews

Each chapter is well written, and provides a sound background understanding together with useful practical details and user experience ... It is a good source of ideas and solutions that will enable many to develop their own highly selective bioseparations. - The Genetic Engineer and Biotechnologist; This is a well put-together and presented book - highly practical in nature...Four stars. - Australian Biotechnology Association

Each chapter is well written, and provides a sound background understanding together with useful practical details and user experience ... It is a good source of ideas and solutions that will enable many to develop their own highly selective bioseparations. - The Genetic Engineer and Biotechnologist; This is a well put-together and presented book - highly practical in nature...Four stars. - Australian Biotechnology Association

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