Overview

Recent advances in array-based detectors and imaging technologies have provided high throughput systems that can operate within a substantially reduced timeframe and other techniques that can detect multiple contaminants at one time. These technologies are revolutionary in terms of food safety assessment in manufacturing, and will also have a significant impact on areas such as public health and food defence. This book summarizes the latest research and applications of sensor technologies for online and high throughput screening of food. The book first introduces high throughput screening strategies and technology platforms, and discusses key issues in sample collection and preparation. The subsequent chapters are then grouped into four sections: Part I reviews biorecognition techniques; Part II covers the use of optical biosensors and hyperspectral imaging in food safety assessment; Part III focuses on electrochemical and mass-based transducers; and finally Part IV deals with the application of these safety assessment technologies in specific food products, including meat and poultry, seafood, fruits and vegetables.

Full Product Details

Author:   Arun K. Bhunia (Arun K. Bhunia, Purdue University, USA) ,  Moon S. Kim (USDA-ARS Beltsville, USA) ,  Chris R. Taitt (Naval Research Laboratory, Washington DC, USA)
Publisher:   Elsevier Science & Technology
Imprint:   Woodhead Publishing Ltd
Dimensions:   Width: 15.20cm , Height: 2.80cm , Length: 22.90cm
Weight:   0.730kg
ISBN:  

9780081013830

ISBN 10:   0081013833
Pages:   550
Publication Date:   19 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

List of contributors Woodhead Publishing Series in Food Science, Technology and Nutrition 1. High throughput screening strategies and technology platforms for detection of pathogens: an introduction Abstract 1.1 Introduction 1.2 Current detection strategies 1.3 Why high throughput screening (HTS) is needed 1.4 HTS technologies for foodborne pathogens – present and future trends 2. Sampling and sample preparation for sensor-based detection of pathogens in foods Abstract 2.1 Introduction 2.2 Key issues in sample preparation: from “Farm to Fork to Physician” 2.3 Challenges in sampling from food matrices and on “bulk” surfaces 2.4 Nonspecific vs. specific methods 2.5 Physical methods 2.6 Chemical and combined methods 2.7 Capture and concentration of whole microbial cells 2.8 The use of cleaning materials in sampling 2.9 Capture and concentration of pathogen DNA from complex food matrices 2.10 Innovations in selective enrichment strategies 2.11 Conclusions Part One: Biorecognition techniques 3. Antibodies, enzymes, and nucleic acid sensors for high throughput screening of microbes and toxins in food Abstract 3.1 Introduction 3.2 Conventional methods for bacterial pathogen detection 3.3 Rapid and advanced technologies 3.4 Antibody structure and production 3.5 Polyclonal and monoclonal antibodies for biorecognition 3.6 The identification of recombinant antibodies by phage display technology 3.7 Biopanning of phage display libraries 3.8 Biosensors and antibody immobilization strategies 3.9 Immunosensor-based applications for high throughput pathogen screening 3.10 Multiplexed pathogen detection using antibodies for biorecognition 3.11 Nucleic acid assays 3.12 Microarray-based technologies 3.13 Enzyme-based sensors 3.14 High throughput bacterial toxin detection 3.15 High throughput fungal pathogen and mycotoxin detection 3.16 Marine toxins 3.17 Selected commercial platforms for high throughput detection 3.18 Conclusion 4. Phage technology in high throughput screening for pathogen detection in food Abstract Acknowledgments 4.1 Introduction 4.2 Pathogen detection using phage: culture-based methods and phage typing 4.3 Pathogen detection using phage: phage-host adhesion-based methods 4.4 Pathogen detection using phage: biosensors 4.5 Pathogen detection using phage: phage-triggered ion cascade 4.6 Pathogen detection using phage: phage replication and metabolism-based methods 4.7 Pathogen detection using phage: phage lysis-based methods 4.8 Conclusion 5. Mammalian cell-based sensors for high throughput screening for detecting chemical residues, pathogens, and toxins in food Abstract Acknowledgments 5.1 Introduction 5.2 The need for novel methods in food control 5.3 Cell-based biosensors for food safety 5.4 Mammalian cell-based biosensors 5.5 Robustness and shelf life of mammalian cell-based biosensors 5.6 Conclusions and future trends Part Two: Optical transducers and hyperspectral imaging 6. Label-free light-scattering sensors for high throughput screening of microbes in food Abstract Acknowledgments 6.1 Introduction 6.2 Elastic light-scattering-based high throughput screening of microorganisms 6.3 Application of BARDOT-based high throughput screening in food safety 6.4 Future trends 7. Vibrational spectroscopy for food quality and safety screening Abstract Acknowledgments 7.1 Introduction 7.2 Basic concepts of vibrational spectroscopy 7.3 Applications in food quality 7.4 Applications in food safety 7.5 Hyperspectral imaging for food quality and safety 7.6 Summary and future trends 8. Flow cytometry and pathogen screening in foods Abstract Acknowledgments 8.1 Introduction 8.2 Analysis of foods using classical flow cytometry 8.3 Analysis of foods using bead-based detection 8.4 Future trends 8.5 Conclusions 9. Fluorescence-based real-time quantitative polymerase chain reaction (qPCR) technologies for high throughput screening of pathogens Abstract Acknowledgments 9.1 Introduction 9.2 Basics of real-time qPCR 9.3 Pre-PCR processing 9.4 Instrumentation for qPCR 9.5 Examples of qPCR for high throughput screening of foodborne pathogens 9.6 Future trends 9.7 Sources of further information and advice 10. Fiber-optic sensors for high throughput screening of pathogens Abstract Acknowledgments 10.1 Introduction 10.2 General view of immunosensors 10.3 Evanescent field optical biosensors 10.4 Fiber-optic probes and immobilization of ligands 10.5 Application of evanescent wave biosensors for detection of foodborne pathogens 10.6 Conclusions and future trends Part Three: Electrochemical and mass-based transducers 11. Electronic noses and tongues in food safety assurance Abstract 11.1 Introduction 11.2 Functioning of electronic noses and tongues 11.3 Food safety applications of electronic noses 11.4 Food safety applications of electronic tongues 11.5 Conclusions and future trends 12. Impedance microbiology and microbial screening strategy for detecting pathogens in food Abstract 12.1 Introduction 12.2 Impedance for microbiological testing 12.3 Standard impedance 12.4 Specific applications for testing food 12.5 Advantages and disadvantages of impedance testing 12.6 Summary and future trends 13. Immunologic biosensing of foodborne pathogenic bacteria using electrochemical or light-addressable potentiometric sensor (LAPS) detection platforms Abstract 13.1 Introduction 13.2 Immunoelectrochemistry (IEC) 13.3 Using IEC to detect pathogenic bacteria 13.4 Improving cell capture in IEC and applications in food screening 13.5 Light-addressable potentiometric sensing 13.6 Future trends 13.7 Sources of further information and advice 14. Conductometric biosensors for high throughput screening of pathogens in food Abstract 14.1 Introduction 14.2 Biosensors 14.3 Conductometric biosensors and gas sensors 14.4 Conductometric biosensors: general and food safety applications 14.5 Future trends and conclusions 15. Microfluidic biosensors for high throughput screening of pathogens in food Abstract 15.1 Introduction 15.2 Microfluidics 15.3 Immunoassays for pathogen sensing using monoclonal, polyclonal, and recombinant antibodies 15.4 Alternatives to antibodies: immunoassays using molecular imprinted polymers, molecular probes, and aptamers 15.5 Microfluidic immunoassays for detecting foodborne pathogens 15.6 Microfluidic techniques using nucleic acid (NA) analysis 15.7 Lab-on-a-chip (LOC) platforms for NA foodborne pathogen detection 15.8 Microfluidic food processing: sample preparation, isolation, and amplification 15.9 Integrated LOC devices for high throughput screening 15.10 Conclusion 16. Magnetoelastic sensors for high throughput screening of pathogens in food Abstract 16.1 Introduction 16.2 Freestanding magnetoelastic (ME) biosensors 16.3 Fabrication of ME biosensors 16.4 Biomolecular recognition elements used on ME biosensors 16.5 Interrogation system for ME biosensors 16.6 Applications of ME biosensors as a foodborne screening technique 16.7 Potential applications of the ME biosensor technique along the food chain 16.8 Conclusions Part Four: Specific applications 17. Total internal reflection fluorescence (TIRF) array biosensors for biothreat agents for food safety and food defense Abstract Acknowledgments 17.1 Introduction: waveguides, total internal reflection, and the evanescent wave 17.2 Planar waveguide TIRF array biosensors 17.3 Planar waveguide TIRF arrays in food analysis 17.4 Commercial TIRF array technologies 17.5 Array biosensors for food defense 17.6 Future directions 17.7 Conclusions 18. Online screening of meat and poultry product quality and safety using hyperspectral imaging Abstract Acknowledgments 18.1 Introduction 18.2 Fundamentals of hyperpsectral imaging 18.3 The role of spectral techniques in online screening of food 18.4 Implementation of online spectral screening systems for evaluating meat quality 18.5 Key stages in online spectral screening systems 18.6 Using hyperspectral imaging to measure individual meat quality attributes 18.7 Measuring quality in beef and pork 18.8 Measuring quality in lamb, chicken, and turkey 18.9 Measuring quality in fish 18.10 Using hyperspectral imaging to identify bacteria and other types of contaminants 18.11 Using hyperspectral imaging to authenticate meat and meat products 18.12 Conclusions and future trends 19. Online screening of fruits and vegetables using hyperspectral line-scan imaging techniques Abstract Acknowledgments 19.1 Introduction 19.2 Line-scan hyperspectral imaging techniques 19.3 Quality and safety evaluation of fruits and vegetables 19.4 Animal fecal contamination on produce 19.5 Hyperspectral/multispectral imaging for online applications 19.6 Whole-surface online inspection of fruits and leafy greens 19.7 Conclusions 20. High throughput screening of seafood for foodborne pathogens Abstract 20.1 Introduction 20.2 Seafood pathogens and products 20.3 Standard methods 20.4 Nucleic acid-based methods 20.5 Nucleic acid hybridization 20.6 Antibody-based methods 20.7 Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry 20.8 Infrared (IR) spectroscopy 20.9 High throughput screening systems for seafood pathogens 20.10 Future trends 20.11 Additional information Index

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Author Information

Arun K. Bhunia is a Professor of Food Microbiology at Purdue University, USA Moon S. Kim is a research physicist with the Agricultural Research Service, USDA, USA Chris R. Taitt is a research biochemist at the Naval Research Laboratory, Washington DC, USA

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