High-Throughput Next Generation Sequencing: Methods and Applications

Author:   Young Min Kwon ,  Steven C. Ricke
Publisher:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2011
Volume:   733
ISBN:  

9781493961641


Pages:   308
Publication Date:   23 August 2016
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
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High-Throughput Next Generation Sequencing: Methods and Applications


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Author:   Young Min Kwon ,  Steven C. Ricke
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2011
Volume:   733
Weight:   0.829kg
ISBN:  

9781493961641


ISBN 10:   1493961640
Pages:   308
Publication Date:   23 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Helicos Single Molecule Sequencing of Bacterial Genomes.- Whole Genome Sequencing of Unculturable Bacterium Using Whole Genome Amplification.- RNA Sequencing and Quantitation Using the Helicos Genetic Analysis System.- Transcriptome Profiling Using Single-Molecule Direct RNA Sequencing.- Discovery of Bacterial sRNAs by High-Throughput Sequencing.- Identification of Virus Encoding MicroRNAs Using 454 FLX Sequencing Platform.- Ribosomal RNA Depletion for Massively Parallel Bacterial RNA-Sequencing Applications.- Integrating High-Throughput Pyrosequencing and Quantitative Real-Time PCR to Analyze Complex Microbial Communities.- Tag-Encoded FLX Amplicon Pyrosequencing for the Elucidation of Microbial and Functional Gene Diversity in Any Environment.- Pyrosequencing of Chaperonin-60 (cpn60) Amplicons as a Means of Determining Microbial Community Composition.- Prescreening of Microbial Populations for the Assessment of Sequencing Potential.- Metagenomics.- Metagenomic Analysis of Intestinal Microbiomes in Chickens.- Gene Expression Profiling: Metatranscriptomics.- High-Throughput Insertion Tracking by Deep Sequencing for the Analysis of Bacterial Pathogens.- Determining DNA Methylation Profiles Using Sequencing.- Preparation of Next-Generation Sequencing Libraries Using Nextera™ Technology: Simultaneous DNA Fragmentation and Adaptor Tagging by In Vitro Transposition.- Amplification-Free Library Preparation for Paired-End Illumina Sequencing.- Target-Enrichment through Amplification of Hairpin-Ligated Universal Targets for Next-Generation Sequencing Analysis.- 96-Plex Molecular Barcoding for the Illumina Genome Analyzer.

Reviews

From the reviews: This well-illustrated book details the most recent advances in the applications of next generation sequencing technologies with an emphasis on microorganisms and their communities, in addition to general applications relevant to the study of any living organism. ... The book is intended for scientists interested in this area, but even graduate students and other researchers field may find this book useful. (Omer Iqbal, Doody's Book Reviews, March, 2012) There are definitely some highlights that will make this book of use to both NGS Newbies and experienced practitioners. ... many readers will find this a very useful book. (Michael A. Quail, Human Genetics, Vol. 130, 2011)


From the reviews: This well-illustrated book details the most recent advances in the applications of next generation sequencing technologies with an emphasis on microorganisms and their communities, in addition to general applications relevant to the study of any living organism. ... The book is intended for scientists interested in this area, but even graduate students and other researchers field may find this book useful. (Omer Iqbal, Doody's Book Reviews, March, 2012) There are definitely some highlights that will make this book of use to both NGS Newbies and experienced practitioners. ... many readers will find this a very useful book. (Michael A. Quail, Human Genetics, Vol. 130, 2011)


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