Overview

High-performance liquid chromatography (HPLC) has emerged as the most powerful and versatile separation and analytical method. This book covers not only the conventional HPLC techniques but also the new developments, novel separation modes, column technology, as well as procedures and practices, particularly the advanced applications of HPLC in the fields of pharmaceutical, clinical, bioanalytical and food sciences. An in-depth discussion of separation mechanisms and characteristics of stationary and mobile phases is well-blended with practical method development and validation. Practical and pragmatic approaches and actual examples for rapid and effective development of fast, selective, accurate and reliable HPLC methods are provided. This book consists of eleven chapters. The first chapter explains the basic principles of hydrophilic interaction liquid chromatography (HILIC), an appealing alternative HPLC separation mode for separating polar compounds along with the properties of HILIC stationary and mobile phases. The technical aspects and the applications as well as actual HILIC method development are illustrated. The second chapter describes recent progress in HPLC column technology. The advantages and disadvantages of sub-2 m porous particles, solid core particles and monolithic columns over the conventional columns are discussed in terms of the separation efficiency, speed, resolution and applications. Chapters Three to Eleven are devoted to advanced applications of modern HPLC including RP-HPLC, NP-HPLC, Ion chromatography, HILIC, LC-MS/MS in pharmaceuticals, clinical, biomarkers, dietary supplements, food analysis and dye examination in archeological textiles.

Full Product Details

Author:   Yuegang Zuo
Publisher:   Nova Science Publishers Inc
Imprint:   Nova Science Publishers Inc
Dimensions:   Width: 18.00cm , Height: 2.40cm , Length: 26.00cm
Weight:   0.780kg
ISBN:  

9781629488547

ISBN 10:   1629488542
Pages:   312
Publication Date:   01 March 2014
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Available To Order  
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Table of Contents

Preface; Hydrophilic Interaction Liquid Chromatography: Fundamentals & Applications; The New Generation of HPLC Columns: Evolution of Packing Materials; High-Performance Liquid Chromatography for Carbohydrate Analysis; High-Performance Liquid Chromatography for the Analysis of Non-Nucleoside Reverse Transcriptase Inhibitors in Biological Fluids & Pharmaceutical Products; High-Performance Liquid Chromatography: An Effective Tool for Quality Control of Natural Cholesterol-Lowering Agents; Measurement of Cholesterol Levels of Lipoprotein Classes by Using Anion-Exchange Chromatography; Recent Trends in LC Analysis of Biological Materials for Determination of Benzodiazepine Drugs; HPLC Analytical Study of Methacrylic Monomers Released from Dental Composite Materials; HPLC Analysis of Vitamin B1, B2, B3, B6, B9, B12 & Vitamin C in Various Food Matrices; Ultra Performance Liquid Chromatography Coupled to Ultraviolet-Vis & Mass Spectrometry Detector for Screening of Organic Acids & Polyphenols in Red Wine; HPLCUV-VisESI MS Examination of Archeological Fibers - Red Natural Dyes in Italian Textiles from 15th & 16th Centuries; Index.

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