Overview

The porphyrins, chlorophylls, bilins and related tetrapyrroles are vital for all living organisms. Natural and synthetic tetrapyrroles are used extensively in foods, cosmetics, biotechnology, pharmaceuticals, diagnostics and medicine. Methods for their separation and characterization therefore, have a very wide area of applications. Yet, there is a dearth of books dedicated to HPLC and HPLC/MS of tetrapyrroles. Lim addresses this problem admirably by providing practical HPLC and HPLC/MS protocols coupled with in-depth chromatographic and mass spectrometric reference data. These are invaluable in the analysis, identification and characterization of porphyrins, chlorophylls, bilins and other related compounds found in biological and clinical materials. HPLC method development and optimization for coupling to mass spectrometry are also described in rich detail. Sample preparation, and suggestions for avoiding procedural artifacts during extraction of clinical and biological samples are discussed. Clinical biochemists involved in biochemical diagnosis of human porphyrias will find this monograph assuredly helpful, as would analysts, biochemists and chemists involved in the separation, isolation and characterization of natural and synthetic tetrapyrroles. Undoubtedly, Lim has contributed a master-piece containing sufficient background material for beginners and up-to-date references for all researchers in the field.Contents: Structure, Distribution, Biosynthesis and FunctionHigh-Performance Liquid Chromatography of PorphyrinsMass Spectrometry of PorphyrinsPorphyrin Profiles in Blood, Urine and Faeces by HPLC and HPLC/ESI-MSIsolation and Characterization of Protoporphyrin Glycoconjugates from Harderian Glands of Rodents by HPLC and HPLC/ESI-MSHPLC and HPLC/MS of Chlorophyll and Related CompoundsHPLC and HPLC/MS of Bilins of Animal and Plant OriginFuture Directions of HPLC and Mass spectrometry of TetrapyrrolesReadership: Analytical biochemists, clinical biochemists, researchers in tetrapyrolle chemistry and biochemistry, plant scientists, pharmaceutical chemists.

Full Product Details

9781282758247

Table of Contents

Reviews

Author Information

Tab Content 6

Customer Reviews

Recent Reviews

Countries Available