Overview

An outstanding collection of state-of-the-art experimental methods for herpes virology. These procedures range from the more biological in vivo maneuvers to the purely molecular, and are described in detail by selected experts to ensure reproducibility. Most of the protocols rely on the herpes simplex virus as prototype, so that it then becomes relatively easy to extrapolate and make the necessary modifications required for application to other herpesviruses, especially members of the alpha group, such as PRV and EHV.

Full Product Details

Author:   S. Moira Brown ,  Alasdair R. MacLean
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1998 ed.
Volume:   10
Dimensions:   Width: 15.50cm , Height: 2.80cm , Length: 23.50cm
Weight:   1.780kg
ISBN:  

9780896033474

ISBN 10:   0896033473
Pages:   419
Publication Date:   18 November 1997
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

HSV Growth, Preparation, and Assay.- HSV Entry and Spread.- Preparation of HSV-DNA and Production of Infectious Virus.- HSV Mutagenesis.- Saturating Mutagenesis and Characterization of a Herpesvirus Genome Using In Vivo Reconstitution of Virus from Cloned Subgenomic Regions.- Construction and Use of Cell Lines Expressing HSV Genes.- Analysis of HSV Polypeptides.- Protein Purification.- Expression and Purification of Secreted Forms of HSV Glycoproteins from Baculovirus-Infected Insect Cells.- Crystallization of Macromolecules for Three-Dimensional Structure Determination.- Direct Immunogold Labeling of Herpesvirus Suspensions.- Expression of HSV Proteins in Bacteria.- In Vitro Systems to Analyze HSV Transcript Processing.- Analysis of HSV-1 Transcripts by RNA-PCR.- Transient Assays for HSV Origin and Replication Protein Function.- HSV Amplicons in Gene Therapy.- Analyses of HSV Proteins for Posttranslational Modifications and Enzyme Functions.- HSV-Cellular Protein Interactions.- Models of Recurrent Infection with HSV in the Skin and Eye of the Mouse.- Pathogenesis and Molecular Biology of HSV Latency and Ocular Reactivation in the Rabbit.- HSV Latency In Vitro.- Assessing Cell-Mediated Immune Responses to HSV in Murine Systems.- Assays for HSV Gene Expression During Establishment and Maintenance of Latent Infection.- Analysis of HSV-DNA and RNA Using the Polymerase Chain Reaction.- HSV Vectors for Gene Therapy.- Investigation of the Anti-HSV Activity of Candidate Antiviral Agents.

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