Overview

This volume details experimental protocols to study structure and function of hepatocytes in vivo and in vitro, and for characterizing heterogeneity and plasticity of hepatocytes in healthy and in injured liver. Chapters guide readers through separating hepatocyte subfractions, characterizing the heterogeneity, novel culture systems, hepatocyte-like cells from human pluripotent stem cells, analyzing drug metabolism, monitoring protein sorting, manipulating gene expression, and methods for expanding primary hepatoblastoma and identifying hepatocellular carcinoma subtypes. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   Authoritative and cutting-edge, Hepatocytes: Methods and Protocols aims to provide techniques to further understand molecular mechanisms involved in the regulating hepatocyte functions and underlying liver diseases.

Full Product Details

Author:   Naoki Tanimizu
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2544
Weight:   0.758kg
ISBN:  

9781071625569

ISBN 10:   107162556
Pages:   281
Publication Date:   21 September 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1.      Long-Term Expansion of Primary Hepatocyte Organoids Thomas A. Kluiver, Lianne J. Kraaier, and Weng Chuan Peng   2.      Generation of in vivo traceable hepatocyte-like cells from human iPSCs　 Candice Ashmore-Harris and Gilbert O. Fruhwirth   3.      Generation of hepatocyte organoids from human iPS cells Giuseppe Pettinato 4.      Induction of bile canaliculi forming hepatocytes from human induced pluripotent stem cells Lavinija Matakovic, Arend W. Overeem, Karin Klappe, and Sven C. D. van IJzendoorn   5.      Human hepatocyte transduction with adeno-associated virus vector　 Zhenwei Song, Wenwei Shao, Liujiang Song1, Xieolei Pei, and Chengwen Li   6.      Hepatocyte-directed delivery of Lipid-encapsulated small interfering RNA Laura Morán, Marius Maximilian Woitok, Matthias Bartneck, and Francisco Javier Cubero   7.      Co-culture model for hepatitis B virus infection using iPSC-derived liver progenitor cells and liver sinusoidal endothelial cells Misao Himeno, Shin-Wei Chen, and Taketomo Kido   8.      In vitro assay system to detect drug-induced bile acid-dependent cytotoxicity using hepatocytes Akinori Takemura, and Kosei Itoh   9.      Evaluation of urea cycle activity by metabolic flux analysis using mass spectrometry Yasuharu Ueno, Takuji Maeda, Satoshi Okamoto, and Hideki Taniguchi   10.   Live Cell Imaging of Biosynthetic Protein Transport in Hepatocytes　 Francisco Lázaro-Diéguez and Anne Müsch 11.   Collagen sandwich culture of primary hepatocytes for image-based investigations　 Dong Fu 12.   Assessment of Hepatocyte Ploidy by Flow Cytometry Yinhua Jin, Teni Anbarchian, and Roel Nusse   13.   Isolation of small hepatocyte-like progenitor cells from retrorsine/partial hepatectomy rat livers by laser micro-dissection Norihisa Ichinohe, Naoki Tanimizu, and Toshihiro Mitaka   14.   Application of EdU-based DNA synthesis assay to measure hepatocyte proliferation in situ during liver regeneration　　 Amit Ghosh, Awais Ullah Ihsan, Madhuparna Nandi, Maryse Cloutier, Md Gulam Musawwir Khan, Sheela Ramanathan and Subburaj Ilangumaran 15.   Quantification of clonal expansion of hepatocytes in normal and injured liver　 Feng Chen   16.   Evaluation of SOX9-positive hepatocytes in human liver specimens and mature mouse hepatocytes　 Yuji Suzuki, Tokio Sasaki, Keisuke Kakisaka, Hiroaki Abe, and Yasuhiro Takikawa   17.   Identification of genes regulating hepatocyte injury by a genome-wide CRISPR-Cas9 Screen　 Katherine Shortt and Daniel P. Heruth   18.   Identification of subtypes of HCC using bioinformatics and the hepatocyte differentiation model　　 Wei Cheng, Yan Zhang, and Ming Liu   19.   3D culture of primary patient-derived hepatoblastoma tumoroids Peng V. Wu and Roel Nusse   20.   Preparation of functional heaptocytes Ayumu Okumura and Naoki Tanimizu  

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