Overview

A collection of readily reproducible experimental and clinical techniques for studying the molecular basis of hemoglobin disorders. These step-by-step methods utilize a wide variety of the latest analytical techniques, including x-ray crystallography, high performance liquid chromatography, electrophoresis, and nuclear magnetic resonance. Additional methods are offered for prenatal diagnostic analysis, the DNA diagnosis of hemoglobin mutations, hemoglobin fluorescence, and the semisynthesis of hemoglobin.

Full Product Details

Author:   Ronald L. Nagel
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 1st ed. 2003
Volume:   82
Dimensions:   Width: 15.20cm , Height: 1.80cm , Length: 22.90cm
Weight:   0.475kg
ISBN:  

9781617372780

ISBN 10:   1617372781
Pages:   300
Publication Date:   09 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

X-ray Crystallography of Hemoglobins Martin K. Safo and Donald J. Abraham Analysis of Hemoglobins and Globin Chains by High-Performance Liquid Chromatography Henri Wajcman Purification and Molecular Analysis of Hemoglobin by High-Performance Liquid Chromatograpy Belur N. Manjula and Seetharama A. Acharya Oxygen Equilibrium Measurements of Human Red Blood Cells Jean Kister and Henri Wajcman Measurement of Rate Constants for Reactions of O2, CO, and NO with Hemoglobin John S. Olson, Erin W. Foley, David H. Maillett, and Eden V. Paster Electrophoretic Methods for Study of Hemoglobins Henri Wajcman DNA Diagnosis of Hemoglobin Mutations John M. Old. Methods for Analysis of Prenatal Diagnosis, John M. Old Hemoglobin Fluorescence Rhoda Elison Hirsch Nucleation and Crystal Growth of Hemoglobins: The Case of HbC Peter G. Vekilov, Angela Feeling-Taylor, and Rhoda Elison Hirsch Semisynthesis of Hemoglobin Seetharama A. Acharya and Sonati Srinivasulu b-Globin-like Gene Cluster Haplotypes in Hemoglobinopathies Shanmugakonar Muralitharan, Rajagopal Krishnamoorthy, and Ronald L. Nagel Transgenic Mice and Hemoglobinopathies Mary E. Fabry, Eric E. Bouhassira, Sandra M. Suzuka, and Ronald L. Nagel Recombinant Single Globin-Chain Expression and Purification Kazuhiko Adachi Nuclear Magnetic Resonance of Hemoglobins Jonathan A. Lukin and Chien Ho Solubility Measurement of the Sickle Polymer Mary E. Fabry, Seetharama A. Acharya, Sandra M. Suzuka, and Ronald L. Nagel Index

Reviews

...the book is a very good practical volume for those interested in further clinical and experimental studies of hemoglobin disorders. - Biomedical Chromatography

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