Overview

This detailed volume collects updates on the technical advances in hematopoietic stem cell research and incorporates new techniques focused on the molecular/genetic, cellular, and whole organism levels. Exploring methods that apply stress to hematopoiesis, the book also contains chapters focused on better understanding the role of hematopoietic niches and their cellular components, as well as in vivo models that test and quantitate stem cell function and are key to further development of therapeutic applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Hematopoietic Stem Cells: Methods and Protocols serves as a valued addition to laboratories focused on understanding hematopoietic stem cell biology and the therapeutic advances that can be derived from it.

Full Product Details

Author:   Louis M. Pelus ,  Jonathan Hoggatt
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2567
Weight:   0.758kg
ISBN:  

9781071626788

ISBN 10:   1071626787
Pages:   282
Publication Date:   19 October 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

The Factory of Blood Production: Hematopoietic Stem Cells.- Detection of DNA Damage in Hematopoietic Stem Cells.- Evaluating Histone Acetylation in Mouse Hematopoietic Stem and Progenitor Cells Using Chromatin Immunoprecipitation.- CRISPR Gene Editing of Hematopoietic Stem and Progenitor Cells.- Lentiviral Transduction of Nonhuman Primate Hematopoietic Stem and Progenitor Cells.- Identification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells.- Isolation and Characterization of Fetal Liver Hematopoietic Stem Cells.- Utilizing CyTOF to Examine Hematopoietic Stem and Progenitor Phenotype.- Hematopoietic Stem Cell Identification Post-Radiation.- Intravital Microscopy for Hematopoietic Studies.- Laser Micromachining of Bone as a Tool for Studying Bone Marrow Biology.- MSC and HSPC Co-Culture: Mimicking Ex Vivo Bone Marrow Niche.- Isolation of Thymus Stromal Cells from Human and Murine Tissue.- Experimental Models of Mouse and Human Hematopoietic Stem Cell Transplantation.- Hematopoietic Stem and Progenitor Cell Identification and Transplantation in Zebrafish.- Establishing a Murine Model of the Hematopoietic Acute Radiation Syndrome.- Purinergic Signaling and Its Role in Mobilization of Bone Marrow Stem Cells.

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions