Overview

Helicobacter pylori Protocols offers an outstanding collection of state-of-the-art protocols for the identification and molecular manipulation of H. pylori. The authoritative contributors supply detailed and readily reproducible protocols for the culturing of H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H. pylori. They also provide molecular epidemiological techniques, including ribotyping, PCR-RFLP, and RAPD-PCR. These procedures have been developed by leading practitioners to solve the difficult technical problems created by the application of the powerful bacterial genetic and molecular cloning techniques to H. pylori.

Full Product Details

Author:   Christopher L. Clayton ,  Harry L. T. Mobley
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1997 ed.
Volume:   8
Dimensions:   Width: 15.50cm , Height: 2.00cm , Length: 23.50cm
Weight:   1.320kg
ISBN:  

9780896033818

ISBN 10:   0896033813
Pages:   274
Publication Date:   24 March 1997
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Introduction: Medical Significance of H. pylori.- Detection of H. pylori Infection by Biopsy Urease, Histology, and Culture.- Serology and Urea Breath Test in the Diagnosis of H. pylori Infection.- The Detection of H. pylori by the Polymerase Chain Reaction.- PCR for the Detection of H. pylori in Gastric Juice Aspirates and Environmental Water Samples.- In vitro Susceptibility Testing of H. pylori.- Defined Media for H. pylori.- Studies of Respiration in H. pylori.- Use of NMR to Study H. pylori Metabolism.- Isolation of H. pylori Genomic DNA and Restriction Analysis.- Ribosomal RNA Gene Restriction Pattern Analysis (Ribotyping) of H. pylori.- Differentiation of H. pylori Strains Using PCR RFLP.- PCR-DNA Sequence Typing.- H. pylori DNA Fingerprinting Using the Arbitrarily Primed PCR (AP-PCR) or Random Amplified Polymorphic DNA (RAPD) Method.- Molecular Methods for Detecting Ulcerogenic Strains of H. pylori.- H. pylori DNA Transformation by Natural Competence and Electroporation.- Random Mutagenesis of the H. pylori Genome.- Construction of a Genomic Map of H. pylori by Pulsed-Field Gel Electrophoresis (PFGE).- Molecular Characterization of H. pylori Surface Antigens.- Isolation and Characterization of Porins from H. pylori.- Methods for the Identification of H. pylori Host Receptors.- Host Responses to H. pylori.- Nonprimate Animal Models of H. pylori Infection.- The Nonhuman Primate Model for H. pylori Infection.

Reviews

The book was designed to provide up-to-date detailed protocols for the experienced either working in the field of Helicobacter research or wishing to enter the field. The authors share their experience and give advice about how to avoid pitfalls and problems encountered with this organism. The book is recommended for those with a serious interest in microbiology and molecular biology of Helicobacter pylori. - David Graham, MD, Baylor College of Medicine

The book was designed to provide up-to-date detailed protocols for the experienced either working<br> in the field of Helicobacter research or wishing to enter the field. The authors share their experience and give advice about how to avoid pitfalls and problems encountered with this organism. The book is recommended for those with a serious interest in microbiology and molecular biology of Helicobacter pylori. - David Graham, MD, Baylor College of Medicine

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