Overview

This detailed book explores the technical breakthroughs with biophysical and cell biological approaches that have advanced the study of molecular mechanisms underlying the generation and transduction of the hedgehog (HH) signal, indicating the important role of sterols in this pathway. Within its pages, the volume examines techniques involving several key protein components in this pathway, including HH, the signaling ligand; Dispatched, a HH secretion regulator; Patched, the receptor of HH; Smoothened, the signal transducer used to transduce the HH signal across the plasma membrane; and GLI, the transcription factor to turn on HH target genes. Written in the highly successful Methods in Molecular Biology series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Hedgehog Signaling: Methods and Protocols provides researchers in clinical and basic science with a fresh and reliable reference for analyzing HH signal transduction through cell biological, biochemical, and biophysical approaches.

Full Product Details

Author:   Xiaochun Li
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2374
Weight:   0.493kg
ISBN:  

9781071617038

ISBN 10:   1071617036
Pages:   244
Publication Date:   28 September 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Generation and Analysis of Mosaic Spinal Cord Organoids Derived from Mouse Embryonic Stem Cells             Carina Jägers and Henk Roelink   2. Evaluating Hedgehog Acyltransferase Activity and Inhibition Using the Acylation-Coupled Lipophilic Induction of Polarization (Acyl-cLIP) Assay             Sebastian A. Andrei, Thomas Lanyon-Hogg, and Edward W. Tate   3. Analysis of Protein Cholesterylation by Biorthogonal Labeling             Ao Hu, Meng Zhou, and Bao-Liang Song   4. An In Situ Fluorescence Assay for Cholesterol Transporter Activity of the Patched             Wonhwa Cho, Arthur Ralko, and Ashutosh Sharma   5. Imaging of the Ciliary Cholesterol Underling the Sonic Hedgehog Signal Transduction             Tatsuo Miyamoto, Kosuke Hosoba, Silvia Natsuko Akutsu, and Shinya Matsuura   6. Studying Hedgehog Signaling during Mouse Neural Tube Development             Sandii Constable, Kevin White, Bandarigoda Nipunika Somatilaka, and Saikat Mukhopadhyay   7. Measuring and Manipulating Membrane Cholesterol for the Study of Hedgehog Signaling             Maia Kinnebrew, Kristen A. Johnson, Arun Radhakrishnan, and Rajat Rohatgi   8. The Immunofluorescence-Based Detection of Hedgehog Pathway Components in Primary Cilia of Cultured Cells             Shu Yang and Xiaoyan Zheng   9. Analysis of Dispatched Protein Processing and Sonic Hedgehog Ligand Release             Elizabeth Cleverdon, Daniel P. Stewart, and Stacey K. Ogden   10. Expression, Purification, and Structure Determination of Human PTCH1–HH-N Complexes             Xiaofeng Qi, Philip Schmiege, Leticia Esparza, and Xiaochun Li   11. Characterization of Smoothened Phosphorylation and Activation             Jianhang Jia, Yuhong Han, Yong Suk Cho, and Jin Jiang   12. Cell-Based Assays for Smoothened Ubiquitination and Sumoylation             Yuhong Han and Jin Jiang   13. Biochemical Assays to Directly Assess Smoothened Activation by a Conformationally Sensitive Nanobody             Ishan Deshpande and Aashish Manglik   14. Studies of SMOOTHENED Activation in Cell-Free and Reconstituted Systems             Isaac B. Nelson and Benjamin R. Myers   15. Rapid, Direct SMOOTHENED Activity Assays in Live Cells Using cAMP-Based Conformational Sensors             Madison F. Walker and Benjamin R. Myers   16. Light-Induced Differentiation of Forebrain Organoids by NVOC-SAG             Ryuji Misawa and Yoshiho Ikeuchi   17. NanoBRET and NanoBiT/BRET-Based Ligand Binding Assays Permit Quantitative Assessment of Small Molecule Ligand Binding to Smoothened             Paweł Kozielewicz and Gunnar Schulte   18. Measuring Smoothened (SMO)-Mediated Activation of the Gi Protein             Heng Liu and Cheng Zhang   19. Ci/Gli Phosphorylation by the Fused/Ulk Family Kinases             Yuhong Han and Jin Jiang   20. A Method for Orthotopic Transplantation of Lung Cancer in Mice             Alexandra N. Wilson, Baozhi Chen, Xin Liu, Jonathan M. Kurie, and James Kim

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions