Overview

This book aims to be an introduction to haplotype information for both the wet and dry labs. Chapters detail co-barcoding and linked-reads based methods, third generation sequencing based methods, Hi-C based methods, single-cell and Strand-seq, and methods for using haplotype data once obtained. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Haplotyping: Methods and Protocols aims to provide compréhensive and accessible methods to undergraduate, graduate, and established scientists.

Full Product Details

Author:   Brock A. Peters ,  Radoje Drmanac
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2590
Weight:   0.787kg
ISBN:  

9781071628188

ISBN 10:   1071628186
Pages:   298
Publication Date:   07 November 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Interrogating the human diplome: computational methods, emerging applications, and challenges.- Targeted Locus Amplification and Haplotyping.- Full length transcript phasing with third generation sequencing.- Large scale complete sequencing and haplotyping of 1-10 kb DNA molecules using short massively parallel reads.- Accurate sequencing and haplotyping from 10 cells using Long Fragment Read (LFR) Technology.- Low-cost genome-scale phasing with barcode-linked sequencing.- A simple cost-effective method for whole genome sequencing, haplotyping, and assembly.- Read-based phasing and analysis of phased variants with WhatsHap.- Hapcut2, a method for phasing genomes using experimental data.- Determining complete chromosomal haplotypes by mLinker.- Haplotyping-assisted diploid assembly and variant detection with linked-reads.- Chromosome-length haplotypes with StrandPhaseR and Strand-seq.- Gamete-binning to achieve haplotype-resolved genome assembly.- Phasing DNA Methylation.- Analysis of 1276 haplotype-resolved genomes allows characterization of cis- and trans-abundant genes.- Phased genome assemblies.- Haplotype-assisted noninvasive prenatal diagnosis of genetic diseases by massively parallel sequencing of maternal plasma cell-free DNA.  

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