Overview

This book is intended for people interested in plant ecophysiology research or related sciences. It should also be useful for scientists working in forestry or agronomy. Twenty-seven chapters cover a whole range of techniques, from electron microscopy applied to seed germination, flow cytometry applied to different measures, cell cycle studies, field and lab measures related to photosynthesis (from pigments to gas exchange and fluorescence), determination of water-related parameters, and techniques to measure phenolics, total soluble proteins, stress proteins, polyamines, proline, RubisCO activity, ATPases, ABA, and ion interchange. Each chapter includes both physiological aspects of the measured parameter and the chosen reference technique for that parameter. A special emphasis is put on allelopathy research, although the book will be valuable for the study of any kind of stress. All chapters can be read independently, and both expert and inexperienced scientists will be able to take advantage of the handbook.

Full Product Details

Author:   M. J. Reigosa Roger
Publisher:   Springer
Imprint:   Springer
Edition:   2001 ed.
Dimensions:   Width: 21.00cm , Height: 2.50cm , Length: 29.70cm
Weight:   1.840kg
ISBN:  

9780792370536

ISBN 10:   0792370538
Pages:   452
Publication Date:   30 June 2001
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Seed Bioassay and Microscopy in the Study of Allelopathy: Radish and Purslane Responses.- Flow Cytometry: Principles and Instrumentation.- Flow Cytometry: DNA Quantification.- Use of Flow Cytometry to Measure Physiological Parameters.- Flow Cytometry: Cell Cycle.- Mitotic Index.- Determination of Photosynthetic Pigments.- Gas Exchange Techniques in Photosynthesis and Respiration Infrared Gas Analyser.- Use of Oxygen Electrode in Measurements of Photosynthesis and Respiration.- Fluorescence Techniques.- Modulated Fluorescence.- Plant Water Status.- Determination of Water Potential in Leaves.- Determination of Relative Water Content.- Determination of Water Potential in Soils.- Determination of Transpiration Using A Steady-State Porometer.- Stomata Imprints: A New and Quick Method to Count Stomata and Epidermis Cells.- HPLC Techniques - Phenolics.- Protein Content Quantification by Bradford Method.- Two-Dimensional Electrophoresis. Stress Proteins.- Polyamines Determination by TLC and HPLC.- Free Proline Quantification.- Assessment of D-Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (Rubisco) Enzymatic Activity.- ATP Phosphohydrolase Activity.- Root Uptake and Release of Ions.- Radioimmunoassay of Abscisic Acid.- Radiochemical Techniques to Quantify Allelochemicals in Plants.

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