Overview

Full Product Details

Author:   Paul Wassarman (Mount Sinai School of Medicine, Mount Sinai Medical Center, New York, NY, USA) ,  Philippe M. Soriano (Professor of Cell, Developmental and Regenerative Biology and Professor of Oncological Sciences, Icahn School of Medicine at Mount Sinai, NY, USA)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Edition:   2nd ed.
Dimensions:   Width: 15.20cm , Height: 2.80cm , Length: 22.90cm
Weight:   1.000kg
ISBN:  

9780123848826

ISBN 10:   0123848822
Pages:   628
Publication Date:   24 September 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

Section I. Transgenesis 1. Lentivirus transgenesis 2. Germline modification using mouse spermatogonial stem cells 3. Embryonic in vivo electroporation in the mouse Section II. Transposons 4. Current applications of transposons in mouse genetics 5. Functional genomics in the mouse using the Sleeping Beauty transposon system 6. The use of DNA transposons for cancer gene discovery in mice Section III. Recombinases 7. A practical summary of site specific recombination, conditional mutagenesis, and tamoxifen induction of CreERT2 8. A recombineering pipeline to make conditional targeting constructs 9. Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria 10.Genetic fate mapping using site-specific recombinases 11. Mapping cell fate and function using recombinase-based intersectional strategies Section IV. Mutagenesis 12. Genome-wide forward genetic screens in mouse ES cells 13. Gene trap mutagenesis in the mouse 14. A wider context for gene trap mutagenesis 15. Mouse mutagenesis with the chemical supermutagen ENU 16. Phenotype-driven mouse ENU mutagenesis screens 17. Using ENU mutagenesis for phenotype-driven analysis of the mouse Section V. Gene Knockdowns 18. Exploration of self-renewal and pluripotency in ES cells using RNAi 19. Transgenic RNAi applications in the mouse 20. Gene knockdown in the mouse through RNAi 21. In vivo analysis of gene knock-down in tetracycline-inducible shRNA mice 22. The power of reversibility: Regulating gene activities via tetracycline controlled transcription Section VI. Gene Expression Profiling 23. Gene expression profiling of mouse oocytes and preimplantation embryos 24. Interrogating the transcriptome of oocytes and preimplantation embryos 25. Gene expression profiling of mouse embryos with microarrays

Reviews

Author Information

Philippe Soriano,is Professor of Cell, Developmental & Regenerative Biology and Professor of Oncological Sciences, Icahn School of Medicine at Mount Sinai, NY, USA

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions