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Author:   Richard R Burgess ,  Murray P. Deutscher (University of Connecticut Health Center, Farmington, Connecticut, U.S.A.)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Edition:   2nd edition
Volume:   436
Dimensions:   Width: 15.20cm , Height: 3.60cm , Length: 22.90cm
Weight:   1.190kg
ISBN:  

9780123749789

ISBN 10:   0123749786
Pages:   912
Publication Date:   01 December 2009
Audience:   Professional and scholarly ,  Professional and scholarly ,  Professional & Vocational ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Out of stock  
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

1. Why Purify Enzymes? 2. Strategies and Considerations for Protein Purifications (Linn) 3. Bioinformatics in Planning a Protein Purification (Burgess) 4. Preparing a Protein Purification Summary Table (Burgess) 5. Setting up a Laboratory (Deutscher) 6. Buffers: Principle and Practice (Blanchard) 7. Measurement of Enzyme Activity (Harris) 8. Quantitation of Protein (Bailey and Noble) 9. Maintaining Protein Stability (Deutscher) 10. Strategy for Choosing and Expression System (Brondyk) 11. Bacterial Expression Systems (Collart) 12. Yeast Expression Systems (Cregg) 13. Baculovirus-Insect Cell Expression Systems (Jarvis) 14. Mammalian Expression Systems (Geisse and Knopf) 15. Expression of Tagged Proteins (Melhorta) 16. Re-folding of Solubilized Inclusion Body Proetin (Burgess) 17. Preparation of Biological Extract (Grabski)

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