Overview

Growth factors play an important role in the regulation of proliferation and differentiation of specific cell types. This is a guide to studying growth factors, from the search for and characterization of new activities, to ""in vivo"" analysis and molecular biology approaches such as DNA cloning. Although cytokines is included in the list of growth factors, the book avoids protocols relating specifically to cytokines and this volume should be seen as complementary to the volume ""Cytokines: A Practical Approach"" in the same series.

Full Product Details

Author:   Ian McKay ,  Irene Leigh (both at the Department of Experimental Dermatology, London Hospital Medical College)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   119
Dimensions:   Width: 14.50cm , Height: 1.60cm , Length: 23.40cm
Weight:   0.470kg
ISBN:  

9780199633593

ISBN 10:   0199633592
Pages:   272
Publication Date:   04 March 1993
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

1. Types of growth factor activity: detection and characterization of new growth factor activities ; 2. Detection of polypeptide growth factors: application of specific bio-assays and PCR technology ; 3. Induction of DNA synthesis and cell division in quiescent cells in response to small peptide growth factors ; 4. Novel gene transcription in response to growth factors: gene isolation by differential plaque hybridization ; 5. Embryological assays for growth factors ; 6. The role of TGF- in bone growth and bone repair ; 7. Localization of growth factor mRNA in tissue sections by in-situ hybridization ; 8. Detection and characterization of growth inhibitory factors: epidermal inhibitory pentapeptide (EIP) ; 9. Expression of growth factors in microbial systems ; 10. Immunoassays for growth factors ; 11. Determination of growth factor receptor distribution by immunocytochemistry or autoradiography and quantitation by ligand binding or FACS analysis ; Appendices

Reviews

<br> A very clear, comprehensive and compact guide which would be of value to anyone working in the field of cytokine/growth factor (GF) research. This is a most informative and useful book, highly recommended for both established researchers and those just entering the field. --Australia and New Zealand Society for Cell Biology<br>

A very clear, comprehensive and compact guide which would be of value to anyone working in the field of cytokine/growth factor (GF) research. This is a most informative and useful book, highly recommended for both established researchers and those just entering the field. --Australia and New Zealand Society for Cell Biology<br>

Author Information

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