Overview

This volume details applications in molecular biological techniques and focuses on applications to determine the involvement of glycans in virus interactions. Chapters guide readers through glycan analysis, glycan distribution analysis, glycan and lectin microarray, preparation of recombinant viral domain protein, reverse genetics and receptor binding, virus-host interactions and receptor binding, and sialidase: assays and inhibitors. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, Glycovirology: Methods and Protocols aims to be an essential resource for researchers who wish to learn more about glycovirology.  

Full Product Details

Author:   Yasuo Suzuki
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2556
Weight:   0.896kg
ISBN:  

9781071626344

ISBN 10:   1071626345
Pages:   357
Publication Date:   30 September 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Comprehensive cellular glycan profiling of glycoproteins and glycosphingolipids by glycoblotting and BEP methods Hisatoshi Hanamatsu and Jun-ichi Furukawa   2. High-performance liquid chromatography mapping method for the structural analysis of N-glycans at the molecular, cellular, and tissue levels Taiki Saito and Hirokazu Yagi   3. Detection of sialic acids on the cell surface using flow cytometry Hirohito Ishigaki and Yasushi Itoh   4. Lectin Staining to Detect Human and Avian influenza Virus Receptors in the Airway of Nonhuman Primates Misako Nakayama and Yasushi Itoh   5. Preparation of Glycan Arrays Using Glycopeptides Derived from Biomaterials Shin-ichi Nakakita, Yukari Nakakita, Ryohsuke Kurihara, and Jun Hirabayashi   6. Glycan profiling of viral glycoproteins with the lectin microarray Takahiro Hiono and Atsushi Kuno   7. Preparation of recombinant PA endonuclease domain protein of influenza A virus and its application for glycobiology research Takashi Kuzuhara   8. Receptor-binding specificity of influenza viruses Masaki Imai, Kosuke Takada, and Yoshihiro Kawaoka   9. Functional analysis of sulphatide in influenza A virus infection and replication Tadanobu Takahashi, Yuuki Kurebayashi, and Takashi Suzuki   10. Quantification of receptor-association, -dissociation and NA-dependent motility of influenza A particles by biolayer interferometry Erik de Vries, Hongbo Guo, Wenjuan Du, Mengying Liu, Frank J. M. van Kuppeveld and Cornelis A.M. de Haan   11. Receptor-binding assay for avian influenza viruses Takahiro Hiono and Daiki Kobayashi   12. Characterization of influenza virus binding to receptors on isolated cell membranes Mikhail N. Matrosovich and Alexandra S. Gambaryan   13. Characterization of human parainfluenza virus receptor using terminal sialic acid linkage-modified cells Keijo Fukushima, Tadanobu Takahashi, and Takashi Suzuki   14. Solid-phase binding assay for ganglioside-binding of human respiroviruses Takashi Suzuki   15. Evaluation of the glycan-binding and esterase activities of hemagglutinin-esterase-fusion glycoprotein from influenza D virus Hao Song and George F. Gao   16. Roles of glycans and non-glycans on the epithelium and in the immune system in H1-H18 influenza A virus infections Nongluk Sriwilaijaroen and Yasuo Suzuki   17. Roles of sialyl glycans in HCoV-OC43, HCoV-HKU1, MERS-CoV and SARS-CoV-2 infections Nongluk Sriwilaijaroen and Yasuo Suzuki   18. Enzymatic substrates and fluorescence imaging of influenza virus sialidase Yuuki Kurebayashi, Tadanobu Takahashi, and Takashi Suzuki   19. Assays for determining the sialidase activity of influenza viruses and monitoring influenza virus susceptibility to neuraminidase inhibitors Emi Takashita   20. Synthesis and Neuraminidase Inhibitory Activity of Sialic Acid Analogues with Fluoro, Phosphono and Sulfo Functionalities Christopher J. Vavricka, Nongluk Sriwilaijaroen, Yasuo Suzuki and Hiromasa Kiyota   21. Influenza A virus neuraminidase inhibitors Nongluk Sriwilaijaroen, Christopher J. Vavricka, Hiromasa Kiyota, and Yasuo Suzuki

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