Overview

Glycoanalysis Protocols, 2nd ed., makes available to all protein scientists, and particularly those working in today's pharmaceuticals industry, the most advanced and reproducible glycoanalysis techniques. These detailed, up-to-date, and proven analytical methods cover the areas of glycoprotein macromolecular structural analysis, oligosaccharide profiling, lipid conjugate characterization, microorganism structure determination, and proteoglycan function. They also include advanced analytical techniques in biotechnology during the production of recombinant glycoproteins and other therapeutics. These protocols will well serve anyone starting work on the analysis of glycoproteins, as well as experienced investigators seeking to carry their expertise to higher levels of accomplishment.

Full Product Details

Author:   Elizabeth F. Hounsell
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2nd ed. 1998
Volume:   76
Dimensions:   Width: 15.50cm , Height: 1.90cm , Length: 23.50cm
Weight:   1.270kg
ISBN:  

9780896033559

ISBN 10:   0896033554
Pages:   262
Publication Date:   27 February 1998
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Characterization of Protein Glycosylation.- Analytical Methods for the Study of O-GlcNAc Glycoproteins and Glycopeptides.- Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography.- Exoglycosidase Sequencing of N-Linked Glycans by the Reagent Array Analysis Method (RAAM).- HPAEC-PAD Analysis of Monosaccharides Released by Exoglycosidase Digestion Using the Carbopac MA1 Column.- HPLC and HPAEC of Oligosaccharides and Glycopeptides.- Analysis of N- and O-Glycans by Pyridylamination.- Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins.- Analysis of Glycosaminoglycans and Proteoglycans.- The Use of Cell and Organ Culture for the Study of Secreted Mucins.- Purification of Gastrointestinal Mucins and Analysis of Their O-Linked Oligosaccharides.- TLC in Structure and Recognition Studies of Glycosphingolipids.- Strategies for Preliminary Characterization of Novel Amphoteric Glycosphingolipids.- Analysis of the Carbohydrate and Lipid Components of Glycosylphosphatidylinos¡tol Structures.- Conformational Analysis of Biantennary Glycopeptides with a Resonance Energy Transfer Technique.

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