Overview

This second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques. Chapter’s guide readers through methods and principles on primordial germ cells and germline stem cells, however many of these principles can be applied to different types of adult stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Germline Stem Cells: Second Edition aims to present the new findings and techniques that have developed since the first edition.

Full Product Details

Author:   Michael Buszczak
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 2nd ed. 2017
Volume:   1463
Weight:   0.470kg
ISBN:  

9781493981540

ISBN 10:   1493981544
Pages:   229
Publication Date:   14 June 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Analysis of the C. Elegans Germline Stem Cell Pool.- Methods for Studying the Germline of the Human Parasite Schistosoma  Mansoni.- Evaluation of the Asymmetric Division of Drosophila Male Germline Stem Cells.- Live Imaging of the Drosophila Testis Stem Cell Niche.- RNA Isolation from Early Drosophila Larval Ovaries.- Live-Cell Imaging of the Adult Drosophila Ovary using Confocal Microscopy.- Measurement Of Mrna Poly(A) Tail Lengths In Drosophila Female Germ Cells and Germline Stem Cells.- Identification of Germline Stem Cells in Zebrafish.- Primordial Germ Cell Isolation from Xenopus Laevis Embryos.- Single-Cell Lineage Analysis of Oogenesis in Mice.- Visualization and Lineage Tracing Of Pax7+ Spermatogonial Stem Cells in the Mouse.- Transplantation as a Quantitative Assay to Study Mammalian Male Germline Stem Cells.- Mouse Fetal Germ Cell Isolation and Culture Techniques.- Rattus Norvegicus Spermatogenesis Colony Forming Assays.- Identification of Mouse Pirna Pathway Components Using Anti-MIWI2 Antibodies.-  Efficient Induction and Isolation of Human Primordial Germ Cell-Like Cells from Competent Human Pluripotent Stem Cells.

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