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Author:   Dinis Pedro Calado
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2017
Volume:   1623
Weight:   1.135kg
ISBN:  

9781493970940

ISBN 10:   1493970941
Pages:   338
Publication Date:   08 June 2017
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Analysis of the Germinal Center Reaction in Tissue Sections.- Detecting Gene Expression in Lymphoid Microenvironments by Laser Microdissection and Quantitative RT-PCR.- Tracking Plasma Cell Differentiation in Living Mice with 2-Photon Microscopy.- Microanatomical Labeling of Germinal Center Structures for Flow Cytometry Using Photoactivation.- Fate Mapping and Transcript Profiling of Germinal Center Cells by Two-photon Photoconversion.- Intravital Microscopy of T-B Cell Interactions in Germinal Centers.- Identifying Follicular Regulatory T cells by Confocal Microscopy.- Cytokine Expression by T follicular Helper Cells.- Follicular Dendritic Cell Isolation and Loading of Immune Complexes.- Isolation and Characterization of Mouse and Human Follicular Dendritic Cells.- In-vitro-induced  Germinal Center B Cell Culture System.- CRISPR/Cas9 Mediated In Vitro Mutagenesis in GC-like B Cells.- Germinal Center Formation with Retrovirally-transduced B Cells for Determining the Role of Specific Molecules In Vivo.- Characterization of the B Cell Transcriptome by RNA-Binding Proteins using iCLIP.- Flow-Cytometric Method Measuring B Cell Surface Immunoglobulin Avidity.- Somatic Hypermutation and Affinity Maturation Analysis Using the 4-hydroxy-3-nitrophenyl-acetyl (NP) System.- Targeting Gene Function in Germinal Center B Cells: A Practical Approach.- Development of Mouse Model Systems of Germinal Centre Lymphomas.- The AID-Cre-ERT2 Model: A Tool for Monitoring B Cell Immune Responses and Generating Selective Hybridomas.- Determining the Origin of Human Germinal Center B Cell-derived Malignancies.- Tracking B-cell Repertoires and Clonal Histories in Normal and Malignant Lymphocytes.- How to Simulate a Germinal Centre. 

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