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Author:   Marco Barchi ,  Massimo De Felici
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2024
Volume:   2770
Weight:   0.765kg
ISBN:  

9781071636978

ISBN 10:   1071636979
Pages:   288
Publication Date:   15 February 2024
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

Isolation and Purification of Viable PGCs from Mouse Embryos.- Purification and Transfection Methods of Chicken Primordial Germ Cells.- Isolation and In Vitro Propagation of Human Spermatogonial Stem Cells.- Purification by STA-PUT Technique of Male Germ Cells from Single Mouse and RNA-Extraction for Transcriptomic Analysis.- Isolation of Mouse Germ Cells by FACS Using Hoechst 33342 and SYTO16 Double Staining.- Isolation and In Vitro Culture of Germ Cells and Sertoli Cells from Human Fetal Testis.- Human Primordial Germ Cell-Like Cell Induction from Pluripotent Stem Cells by SOX17 and PRDM1 Expression.- Induction of Primordial Germ Cell-Like cells from Rat Pluripotent Stem Cells.- Induction of Meiotic Initiation in Long-Term Mouse Spermatogonial Stem Cells Under Retinoid Acid and Nutrient Restriction Conditions.- Lipofection-Based Delivery of CRISPR/Cas9 Ribonucleoprotein for Gene Editing in Male Germline Stem Cells.- Mouse In Vitro Spermatogenesis on 3D Bioprinted Scaffolds.-Histological and Cytological Techniques to Study Perinatal Mouse Ovaries and Oocytes.- Method of Isolation and In Vitro Culture of Primordial Follicles in Bovine Animal Model.- Generation of iPSC Cell Lines from Patients with Sex Chromosome Aneuploidies.- Data Analysis Pipeline for scRNA-Seq Experiments to Study Early Oogenesis.- Mapping Meiotic DNA Breaks: Two Fully-Automated Pipelines to Analyze Single-Strand DNA Sequencing Data, hotSSDS and hotSSDSextra.- “MeiQuant”: An Integrated Tool for Analyzing Meiotic Prophase I Spread Images.

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