Overview

This current edition explores new tests for genotoxicity testing, along with other well-known techniques. This will further help in our understanding of the genotoxic effects of chemicals. The book has different sections dealing with various assays for gene mutation, chromosomal abnormalities, primary DNA damage, etc. It also delves into plant models, animals and their alternates, as well as in silico approaches for genetic toxicology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Genotoxicity Assessment: Methods and Protocols, Second Edition serves as a highly useful and ready resource for research students and scientists working in regulatory toxicology as well as biomedical, biochemical, and pharmaceutical sciences.

Full Product Details

Author:   Alok Dhawan ,  Mahima Bajpayee
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2nd ed. 2019
Volume:   2031
Weight:   0.743kg
ISBN:  

9781493996483

ISBN 10:   1493996487
Pages:   378
Publication Date:   02 September 2020
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Gene Mutation Assays   1. In Vitro Mouse Lymphoma Cell (L5178Y Tk+/- -3.7.2.C) Forward Mutation Assay             Melissa R. Schisler, Martha M. Moore, and B. Bhaskar Gollapudi   2. Erythrocyte-Based Pig-a Gene Mutation Assay             Jeffrey C. Bemis, Nikki E. Hall, and Stephen D. Dertinger   3. Detection of In Vivo Mutation in the Hprt and Pig-a Genes of Rat Lymphocytes             Vasily N. Dobrovolsky, Joseph G. Shaddock, Roberta A. Mittelstaedt, Daishiro Miura, and Robert H. Heflich   Part II: Assays for Chromosomal Abnormalities   4. In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests             Pasquale Mosesso and Serena Cinelli   5. Dicentric Chromosome Assay (DCA) and Cytokinesis-Block Micronucleus (CBMN) Assay in the Field of Biological Dosimetry             Antonella Testa, Valentina Palma, and Clarice Patrono   6. Chromosomal Aberration Test in Human Lymphocytes             Christian Johannes and Guenter Obe   7. In Vivo Micronucleus Assay in Mouse Bone Marrow             Abhishek Kumar Jain and Alok Kumar Pandey   8. Micronucleus Cytome Assays in Human Lymphocytes and Buccal Cells             Claudia Bolognesi and Michael Fenech   9. Micronucleus Analysis by Flow Cytometry             Azeddine Elhajouji and Pascal Stadelmann   10. Hen’s Egg Test for Micronucleus Induction (HET-MN)             Kerstin Reisinger, Eva Dony, Thorsten Wolf, and Katrin Maul   11. Comparative Genomic Hybridization (CGH) in Genotoxicology             Adi Baumgartner, Veronika Hartleb, and Jim D. Taylor   Part III: Tests for Primary DNA Damage   12. The Comet Assay: Assessment of In Vitro and In Vivo DNA Damage             Mahima Bajpayee, Ashutosh Kumar, and Alok Dhawan   13. The Comet Assay in Human Biomonitoring             Diana Anderson and Julian Laubenthal   14. The Comet Assay in Marine Animals             Patrizia Guidi, Brett P. Lyons, and Giada Frenzilli   15. Multicolour Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers             Julian Laubenthal, Michal R. Gdula, Alok Dhawan, and Diana Anderson   16. Detection of DNA Double Strand Breaks Using Pulse Field Gel Electrophoresis             Vyom Sharma and Vishwa Mohan   17. Detection of UV-Induced Thymine Dimers             Vipin Kumar Yadav, Poorwa Awasthi, and Amit Kumar   Part IV: Assays in Plants and Alternate Animal Models   18. Micronucleus Assay with Tetrad Cells of Tradescantia             Miroslav Mišík, Clemens Pichler, Bernhard Rainer, Armen Nersesyan, Katarina Mišíková, and Siegfried Knasmueller   19. The Wing-Spot and the Comet Tests as Useful Assays for Detecting Genotoxicity in Drosophila             Ricard Marcos and Erico R. Carmona   Part V: In Silico Testing for Genetic Toxicology   20. In Silico Approaches in Predictive Genetic Toxicology             Meetali Sinha, Alok Dhawan, and Ramkrishnan Parthasarathi

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