Overview

Andrew Ward and a team of experienced researchers have brought together a collection of optimized classic and vanguard techniques for the identification and analysis of imprinted genes. Protocols are included for identifying and cloning imprinted genes, for analyzing imprinted gene expression, for the study of DNA methylation and methylation-sensitive DNA-binding proteins, and for examining chromatin structure. There are also methods for the manipulation of mouse embryos to produce monoparental embryos and embryonic stem cells, and for the generation of transgenic mice with BAC, PAC,and YAC constructs. Each technique is described in step-by-step detail to ensure successful results.

Full Product Details

Author:   Andrew Ward
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. Softcover of orig. ed. 2002
Volume:   181
Dimensions:   Width: 15.20cm , Height: 2.00cm , Length: 22.90cm
Weight:   0.664kg
ISBN:  

9781617371646

ISBN 10:   1617371645
Pages:   377
Publication Date:   10 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

Generation of Monoparental Embryos for Investigation into Genomic Imprinting.- Deriving and Propagating Mouse Embryonic Stem Cell Lines for Studying Genomic Imprinting.- Balanced Translocations for the Analysis of Imprinted Regions of the Mouse Genome.- Production of YAC Transgenic Mice by Pronuclear Injection.- A Transgenic Approach to Studying Imprinted Genes.- Methylation-Sensitive Genome Scanning.- Subtraction-Hybridization Method for the Identification of Imprinted Genes.- Identification of Imprinted Loci by Methylation.- Ribonuclease Protection.- Quantitative RT-PCR-Based Analysis of Allele-Specific Gene Expression.- Allele-Specific In Situ Hybridization (ASISH).- RNA-FISH to Analyze Allele-Specific Expression.- Flow Cytometry and FISH to Investigate Allele-Specific Replication Timing and Homologous Association of Imprinted Chromosomes.- Southern Analysis Using Methyl-Sensitive Restriction Enzymes.- A PCR-Based Method for Studying DNA Methylation.- Bisulfite-Based Methylation Analysis of Imprinted Genes.- Direct Analysis of Chromosome Methylation.- In Vitro Methylation of Predetermined Regions in Recombinant DNA Constructs.- In Vitro Methylation of Specific Regions in Recombinant DNA Constructs by Excision and Religation.- Detection of Methyl?Sensitive DNA?Binding Proteins with Possible Involvement in the Imprinting Phenomenon.- Probing Chromatin Structure with Nuclease Sensitivity Assays.- Examining Histone Acetlylation at Specific Genomic Regions.- Purification of the MeCP2/Histone Deacetylase Complex from Xenopus laevis.- Reconstitution of Chromatin In Vitro.- Genomic Imprinting in Plants.

Reviews

...a multi-authored volume in which the methods of gene imprinting are discussed in detail in 25 chapters. The protocols are very detailed with multiple schemes and figures. - Journal of Pediatric Endocrinology and Metabolism

"""...a multi-authored volume in which the methods of gene imprinting are discussed in detail in 25 chapters. The protocols are very detailed with multiple schemes and figures."" - Journal of Pediatric Endocrinology and Metabolism"

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