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Author:   Marco Muzi-Falconi ,  Grant W Brown
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2018
Volume:   1672
Weight:   1.281kg
ISBN:  

9781493984473

ISBN 10:   1493984470
Pages:   663
Publication Date:   23 August 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

"The A-like Faker Assay for Measuring Yeast Chromosome III Stability.- The Chromosome Transmission Fidelity Assay for Measuring Chromosome Loss in Yeast.- Measuring Mutation Rates using the Luria-Delbrück Fluctuation Assay.- Molecular Genetic Characterization of Mutagenesis Using a Highly Sensitized Single-Stranded DNA Reporter System in Budding Yeast.- Analyzing Genome Rearrangements in Saccharomyces cerevisiaen: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;"">.- High Resolution Mapping of Modified DNA Nucleobases using Excision Repair Enzymes.- Integrated Microarray-based Tools for Detection of Genomic DNA Damage and Repair Mechanisms.- Study of UV-induced DNA Repair Factor Recruitment: Kinetics and Dynamics.- Inserting Site-specific DNA Lesions into Whole Genomes.- A qPCR-based Protocol to Quantify DSB Resection.- Alkaline Denaturing Southern Blot Analysis to Monitor Double-strand Break Processing.- Single Molecule Analysis of Resection Tracks.- Mapping DNA Breaks by Next-generation Sequencing.- Genome-wide Profiling of DNA Double-strand Breaks by the BLESS and BLISS Methods.- DNA Replication Profiling using Deep Sequencing.- Quantitative Bromodeoxyuridine Immunoprecipitation Analyzed by High-Throughput Sequencing (qBrdU-seq or QBU).- Strand-specific Analysis of DNA Synthesis and ProteinsAssociation with DNA Replication Forks in Budding Yeast.- Analysis of Replicative Polymerase Usage by Ribonucleotide Incorporation.- Dynamic Architecture of Eukaryotic DNA Replication Forks in vivo, Visualized by Electron Microscopy.- A Molecular Toolbox to Engineer Site-Specific DNA Replication Perturbation.- Single Cell Gel Electrophoresis for the Detection of Genomic Ribonucleotides.- Measuring the Levels of Ribonucleotides Embedded in Genomic DNA.- Mapping Ribonucleotides Incorporated into DNA by Hydrolytic End-Sequencing.- Detection of DNA-RNA Hybrids in vivo.- Analysis of de novo Telomere Addition by Southern Blot.- Assays to Study Repair of Inducible DNA Double Strand Breaks at Telomeres.- Telomerase RNA Imaging in Budding Yeast and Human Cells by Fluorescent in situ Hybridization.- Methods to Study Repeat Fragility and Instability in Saccharomyces cerevisiae.- Quantitative Analysis of the Rates for Repeat-mediated Genome Instability in a Yeast Experimental System.- Measuring Dynamic Behaviour of Trinucleotide Repeat Tracts in vivo in Saccharomyces cerevisiae.- The Detection and Analysis of Chromosome Fragile Sites.- Imaging of DNA Ultrafine Bridges in Budding Yeast.- Detection of Ultra-Fine Anaphase Bridges.- A Chromatin Fiber Analysis Pipeline to Model DNA Synthesis and Structures in Fission Yeast.- Long-term Imaging of DNA Damage and Cell Cycle Progression in Budding Yeast Using Spinning Disk Confocal Microscopy.- The CellClamper: A Convenient Microuidic Device for Time-Lapse Imaging of Yeast.- Characterization of Structural and Configurational Properties of DNA by Atomic Force Microscopy.- Genome-wide Quantitative Fitness Analysis (QFA) of Yeast Cultures.- Rewiring the Budding Yeast Proteome using Synthetic Physical Interactions.- Reporter-based Synthetic Genetic Array Analysis: a Functional Genomics Approach for Investigating Transcript or Protein Abundance Using Fluorescent Proteins in Saccharomyces cerevisiae.- Statistical Analysis and Quality Assessment of ChIP-seq Data with DROMPA.- Quantitative Analysis of DNA Damage Signaling Responses to Chemical and Genetic Perturbations."

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