Genome Editing in Animals: Methods and Protocols

Author:   Izuho Hatada
Publisher:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2017
Volume:   1630
ISBN:  

9781493983933


Pages:   256
Publication Date:   08 August 2018
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

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Genome Editing in Animals: Methods and Protocols


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Author:   Izuho Hatada
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2017
Volume:   1630
Dimensions:   Width: 17.80cm , Height: 1.40cm , Length: 25.40cm
Weight:   0.515kg
ISBN:  

9781493983933


ISBN 10:   1493983938
Pages:   256
Publication Date:   08 August 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Construction and Evaluation of Zinc Finger Nucleases.- Current Overview of TALEN Construction Systems.- CRISPR/Cas9.- Computational Prediction of CRISPR/Cas9 Target Sites Reveals Potential Off-Target Risks in Human and Mouse.- Genome Editing of Mouse by Cytoplasmic Injection.- Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing sgRNA/Cas9 Expressing Plasmids.- Genome Editing in Mouse and Rat by Electroporation.- Generation of Knock-in Mouse by Genome Editing.- Genome Editing of Rat.- Gene Targeting in Rabbits: Single-step Generation of Knockout Rabbits by Microinjection of CRISPR/Cas9 Plasmids.- Genome Editing of Pig.- Genome Editing of Monkey.- Genome Editing Mediated by Primordial Germ Cell in Chicken.- CRISPR/Cas9-mediated Targeted Knock-in of Exogenous Reporter Genes in Zebrafish.- Genome Editing of Medaka.- A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis Founders using the CRISPR-Cas System.- Genome Editing of Silkworms.- Genome Editing in the Cricket, Gryllus bimaculatus.-  Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease.- Genome editing of C. elegans.

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